Screening and characterization of fructosyltransferases along with testing different acceptors for achieving maximum transfructosylation activity for producing oligosaccharides-connected new compounds with prebiotic properties.
In particular, the research is focused on producing new alternative sweeteners with improved properties and healthy features from sucrose. The intended approach is to modify commercial sweeteners via enzymatic glycosidation action of Fructosyltranferases using sucrose as fructosyl donor. Thereby, a fructosyl residue from sucrose in transferred to a strong sweetener e.g. sucralose potentially leading to blends of fructooligosaccharide-connected sweeteners with prebiotic and low caloric properties and potential prebiotic oligosaccharides.