Hormonal Regulation of Plant Development (Bio-BB 26)

Course content

Practical course: Students will learn advanced methods in plant biochemistry and plant molecular biology:

• Extraction of total RNA and mRNA; detection of transcripts (competitive RT-PCR, Real Time PCR, in situ hybridization)
• Heterologous gene expression and functional detection of proteins (enzymes and receptors), (protein)-HPLC
• use of bioinformatic tools for plant biochemistry and plant molecular biology
• search and usage of "public domain" databases relevant for plant biochemistry and plant molecular biology (analysis and interpretation of sequence data, development of cloning strategies, primer design, etc.)
• Quantitative Real Time PCR