Course content
After completing the module, students are able to:
- deeply understand the methods of gene transfer and to apply them routinely.
- genetically modify plant and fungal cells using direct and indirect DNA-transfer
- independently analyse successful expression of foreign genes on RNA and protein level and describe it on the level of a changed phenotype.
- independently carry out enzyme kinetic assays of reporters (in vivo and in vitro)
- detect expression of foreign genes using light- and confocal laser scanning microscopy (cLSM)
- differentiate between fluorescence proteins via cLSM, record and edit independently Z-stacks and time videos
- independently generate, document and interpret experimental data.
- discuss critically ways of generating knowledge in a social context.
- understand and independently apply different research strategies.
- plan, give and defend a scientific talk.
- ask scientific-critical questions and discuss about them.
