Dr. Maren Schubert

Maren Schubert
Maren Schubert

Research group leader

Room: 106
Phone: +49.531.391.5760
Fax: +49.531.391.5763
e-mail: maren.schubert@tu-braunschweig.de/m.bleckmann@tu-braunschweig.de

Research interests

  • Development of medications against pandemic threats, e.g. SARS-CoV-2
  • Baculovirus-free generation of human antibodies and difficult to express target proteins in insect cells
  • Recombinant monoclonal antibody development, engineering and characterization
  • VLP (virus like particles) technologies for drug development

Curriculum vitae

10.2018-ongoing        Cand. habil. at TU Braunschweig, Department of Biotechnology

09.2019-04.2020        maternal leave

09.2016-10.2018        Head of Recombinant Protein Production Facility at Rudolf Virchow Centre Würzburg

08.2012-08.2016        PhD student at Helmholtz Centre for Infection Research, “Optimisation of recombinant protein expression in insect cells” in the group of Recombinant Protein Expression

10.2010-07.2012        Master of Science Biotechnology at TU Braunschweig, master thesis „Screening nach stabilen Intrabodies in Säugetierzellen” at the Department of Biotechnology

10.2007-09.2010        Bachelor of Science Biotechnology at TU Braunschweig, bachelor thesis „Biochemische Analyse von SO2 begasten Arabidopsis thaliana“;

 

Fellowships and awards

  • Innovationspreis Niedersachsen (as member of the Corona Antibody Team, 2020)
  • Animal Cell Technology Industry Platform (ACTIP) Fellowship (2015)
  • Bursary of the 24th European Society for Animal Cell Technology Meeting (2015)

Patents

Europäisches Patentamt: Michael Hust, Stefan Dübel, Doris Meier, Federico Bertoglio, Maren Schubert, Stephan Steinke, Marlies Becker, Viola Fühner, Maximilian Ruschig, Philip Alexander Heine, Kai-Thomas Schneider, Rico Ballmann, Susanne Zock-Emmenthal, Kristian Daniel Ralph Roth, Nora Langreder (2020) “Inhibiting anti-SARS-CoV-2 antibodies” (patent filed & outlicensed)

 

Publications

(24)         Jaron, M., Lehky, M., Zarà, M., Zaydowicz, C.N., Lak, A., Ballmann, R., Heine, P.A., Wenzel, E.V., Schneider, K.-T., Bertoglio, F., Kempter,  S., Köster, R.W., Barbieri, S.S., Van den Heuvel, J., Hust, M., Dübel, S., & Schubert, M. (2022). Baculovirus-Free SARS-CoV-2 Virus Like Particle Production in Insect Cells for Rapid Neutralization Assessment. Viruses 14: 2087

(23)         Geisen, U.M., Rose, R., Neumann, F., Ciripoi, M., Vullriede, L., Reid, H.M., Berner, D.K., Bertoglio, F., Hoff, P., Hust, M., Longardt, A.C., Lorentz, T., Martini, G.R., Saggau. C. ,Schirmer, J.H., Schubert, M., Sümbül, M., Tran, F., Voß. M., Zeuner. R., Morrison, P.J., Bacher, P., Fickenscher, H., Gerdes. S., Peipp, M., Schreiber, S., Krumbholz, A. & Hoyer, B.F.  (in press). The long term vaccine-induced anti-SARS-CoV-2 immune response is impaired in quantity and quality under TNFα blockade. J Med Virol

(22)           Cokarić Brdovčak, M., Materljan, J., Šustić, M., Ravlić, S. , Ružić, T., Lisnić, B., Miklić, K., Brizić, I., Pribanić Matešić, M., Juranić Lisnić, V., Halassy, B., Rončević, D., Knežević, Z., Štefan, L., Bertoglio, F., Schubert, M., Čičin-Šain, L., Markotić, A., Jonjić, S. & Krmpotić, A. (2022). ChAdOx1-S adenoviral vector vaccine applied intranasally elicits superior mucosal immunity compared to the intramuscular route of vaccination. Eur J Immunol 52:936-945

(21)         Ballmann, R., Hotop, S.-K., Bertoglio, F., Steinke, S., Heine, P.A., Chaudhry, M.-Z, Jahn, D., Pucker, B., Baldanti, F., Piralla, A., Schubert, M., Čičin-Šain, L., Brönstrup, M., Hust, M. & Dübel, S. (2022). ORFeome phage display reveals a major immunogenic epitope on the S2 subdomain of SARS-CoV-2 Spike protein. Viruses 14: 1326

(20)         Zuo, F., Abolhassani, H., Du, L., Piralla, A., Bertoglio, F., de Campos-Mata, L., Wan, H., Schubert, M., Wang, Y., Sun, R., Cassaniti, I., Vlachiotis, S., Kumagai-Braesch, M., Andréll, J., Zhang, Z., Xue, Y., Wenzel, E.V., Calzolai, L., Varani, L., Rezaei, N., Chavoshzadeh, Z., Baldanti F., Hust, M., Hammarström, L., Marcotte, H. & Pan-Hammarström, Q. (2022). Heterologous immunization with inactivated vaccine followed by mRNA booster elicits strong humoral and cellular immune responses against the SARS-CoV-2 Omicron variant. Nature Communications 13:2670

(19)       Schubert, M., Bertoglio, F., Steinke, S., Heine, P.A., Ynga-Durand, M.A., Maass, H., Sammartino, J.C., Cassaniti, I., Zuo, F., Du, L., Korn, J., Milošević, M., Wenzel, E.V., Krstanović, F., Polten, S., Pribanić-Matešić, M., Brizić, I., Baldanti, F., Hammarström, L., Dübel, S., Šustić, A., Marcotte, H., Strengert, M., Protić, A., Piralla, A., Pan-Hammarström, Q., Čičin-Šain, L. & Hust, M. (2022). Human serum from SARS-CoV-2 vaccinated and COVID-19 patients shows reduced binding to the RBD of SARS-CoV-2 Omicron variant. BMC Medicine 20:102

(18)       Sevenich, M., Thul, E., Lakomek, N.-A., Klünemann, T., Schubert, M., Bertoglio, F., van den Heuvel, J., Petzsch, P., Mohrlüder, J., Willbold, D., (2022). Phage Display-Derived Compounds Displace hACE2 from Its Complex with SARS-CoV-2 Spike Protein. Biomedicines 10: 441.

(17)       Pribanić Matešić, M., Kučan Brlić, P., Lenac Roviš, T., Mačak Šafranko, Ž., Chaouat, A.E., Miklić, K., Malić, S., Ivanković, N., Schubert, M., Bertoglio, F., Markotić, A., Mandelboim, O., Jonjić, S., Brizić, I., (2022). Collection of Monoclonal Antibodies Targeting SARS-CoV-2 Proteins. Viruses 14: 443.

(16)     Marcotte, H., Piralla, A., Zuo, F., Du, L., Cassaniti, I., Wan, H., Kumagai-Braesh, M., Andréll, J., Percivalle, E., Sammartino, J.C., Wang, Y., Vlachiotis, S., Attevall, J., Bergami, F., Ferrari, A., Colaneri. M., Vecchia, M., Sambo, M., Zuccaro, V., Asperges, E., Bruno, R., Oggionni, T., Meloni, F., Abolhassanni, H., Bertoglio, F., Schubert, M., Calzolai, L., Varani, L., Hust, M., Xue, Y., Hammarström, L., Baldanti. F. & Pan-Hammarström, Q. (2021). Immunity to SARS-CoV-2 up to 15 months after infection. iScience 25:103743

(15)      Taubert, R., Engel, B., Diestelhorst, J., Hupa-Breier, K.L., Behrendt, P., Baerlecken, N.T., Sühs, K.W., Janik, M.K., Zachou, K., Sebode, M., Schramm, C., Londoño, M.C., Habes, S., UK-AIH Consortium, Oo, Y.H., Lalanne, C., Pape, S., Schubert, M., Hust, M., Dübel, S., Thevis, M., Jonigk, D., Beimdiek, J., Buettner, F.F.R., Drenth, J.P., Muratori, L., Adams, D.H., Dyson, J.K., Renand, A., Graupera, I., Lohse, A.W., Dalekos, G.N., Milkiewicz, P., Stangel, M., Maasoumy, B., Witte, T., Wedemeyer, H., Manns, M.P. & Jaeckel E. (2021). Quantification of polyreactive immunoglobulin G facilitates the diagnosis of autoimmune hepatitis. Hepatology 75:13-27

(14)      Roth, K.D.R., Wenzel, E.V., Ruschig, M., Steinke, S., Langreder, N., Heine, P.A., Schneider, K.-T., Ballmann, R., Fühner, V., Kuhn, P., Schirmann, T., Frenzel, A., Dübel. S., Schubert, M., Moreira, G.M.S.M., Bertoglio, F., Russo, G. & Hust, M. (2021). Developing recombinant antibodies by phage display against infectious diseases and toxins for diagnostics and therapy. Frontiers Cellular and Infection Microbiology 11:697876

(13)      Bertoglio, F., Fühner. V., Ruschig, M., Heine, P.A., Abasi, L., Klünemann, T., Rand, U., Meier, D., Langreder, N., Steinke, S., Ballmann, R., Schneider, K.-T., Roth, K.D.R., Kuhn, P., Riese, P., Schäckermann, D., Korn, J., Koch, A., Chaudhry, M.Z., Eschke, K., Kim, Y., Zock-Emmenthal, S., Becker, M., Scholz, M., Moreira, G.M.S.G., Wenzel, E.V., Russo, G., Garritsen, H.S.P., Casu, S., Gerstner, A., Roth, G., Adler, J., Trimpert, J., Hermann, A., Schirrmann, T., Dübel, S., Frenzel, A., Van den Heuvel, J., Čičin-Šain, L., Schubert, M. & Hust, M. (2021). A SARS-CoV-2 neutralizing antibody selected from COVID-19 patients is binding to the ACE2-RBD interface and is tolerant to most known RBD mutations. Cell Reports 36: 109433

(12)      Schubert, M., Nimtz, M., Bertoglio, F., Schmelz, S., Lukat, P. & van den Heuvel J. (2021). Reproducible and Easy Production of Mammalian Proteins by Transient Gene Expression in High Five Insect Cells. Methods Mol Biol Clifton NJ 2305: 129–140.

(11)      Sherina, N., Piralla, A., Du, L., Wan, H., Kumagai-Braesh, M., Andréll, J., Braesch-Andersen, S., Cassaniti, I., Percivalle, E., Sarasini, A., Bergami, F., Di Martino, R., Colaneri, M., Vecchia, M., Sambo, M., Zuccaro, V., Bruno, R., Oggionni, T., Meloni, F., Abolhassani, H., Bertoglio, F., Schubert, M., Byrne-Steele, M., Han, J., Hust, M., Xue, Y., Hammarström, L., Baldanti, F., Marcotte, H. & Pan-Hammarström, Q. (2021). Persistence of SARS-CoV-2 specific B-and T-cell responses in convalescent COVID-19 patients 6-8 months after the infection. Med 2: 281-295

(10)      Bertoglio, F., Meier, D., Langreder, N., Steinke, S., Rand, U., Simonelli, L., Heine, P. A., Ballmann, R., Schneider, K.-T., Roth, K. D. R., Ruschig, M., Riese, P., Eschke, K., Kim, Y., Schaeckermann, D., Pedotti, M., Kuhn, P., Zock-Emmenthal, S., Wöhrle, J., Becker, M., Grashoff, M., Wenzel, E. V., Russo, G., Kröger, A., Brunotte, L., Ludwig, S., Fühner, V., Krämer, S. D., Dübel, S., Varani, L., Roth, G., Cicin-Sain, L., Schubert, M., & Hust, M. (2021) SARS-CoV-2 neutralizing human recombinant antibodies selected from pre-pandemic healthy donors binding at RBD-ACE2 interface. Nature Communications 12:1577

(9)        Korn, J., Schäckermann, D., Kirmann, T., Bertoglio, F., Steinke, S., Heisig, J., Ruschig, M., Rojas, G., Langreder, N., Wenzel, E.V., Roth, K.D.R., Becker, M., Meier, D., van den Heuvel, J., Hust, M., Dübel, S. & Schubert, M. (2020). Baculovirus-free insect cell expression system for high yield antibody and antigen production. Scientific Reports 10: 21393

(8)        Behring, S., Hänsch, R., Helmsing, S., Schirrmann, T. & Schubert, M. (2020). Screening for scFv- fragments that are stable and active in the cytosol. Hum. Antibod. doi:10.3233/HAB-200402

(7)        Wenzel, E.V., Bosnak, M., Tierney, R., Schubert, M., Brown, J., Dübel, S., Efstratiou, A., Sesardic, D., Sticking, P. & Hust, M. (2020). Human antibodies neutralizing diphtheria toxin in vitro and in vivo. Scientific Reports 10: 571

(6)        Bleckmann, M., Schürig, M., Endres, M., Samuels, A., Gebauer, D., Konisch, N. & Van den Heuvel, J. (2019). Identifying parameters to improve the reproducibility of transient expression in High Five cells. PLoS ONE; 14(6):e0217878

(5)        de Groot, C., Müsken, M., Bleckmann, M., Ebensen, T., Guzman, C.A. & Müller-Goymann, C.C. (2019). Novel colloidal associations of soyasaponins and lipid components (DPPC, cholesterol) as potential adjuvants for vaccines. Vaccine 2019; 37: 4975–4986

(4)        Karste, K., Bleckmann, M. & van den Heuvel, J. (2017). Not Limited to E. coli: Versatile Expression Vectors for Mammalian Protein Expression. Methods Mol Biol Clifton NJ 1586: 313–324

(3)        Bleckmann, M., Schmelz, S., Schinkowski, C., Scrima, A. & Van den Heuvel, J. (2016). Fast plasmid based protein expression analysis in insect cells using an automated SplitGFP screen. Biotechnology and Bioengineering 113(9):1975-83

[2]        Bleckmann, M., Schürig, M., Chen, F., Yen, Z., Lindemann, N., Meyer, S., Spehr, J. & Van den Heuvel, J. (2016). Identification of essential baculoviral genetic elements for recombinant protein expression levels by transactivation in Sf21 insect cells. PLOS ONE 1(3):e0149424

[1]        Bleckmann, M., Fritz, M., Bhuju, S., Jarek, M., Schürig, M., Geffers, R., Benes, V., Besir, H. & Van den Heuvel, J. (2015). Genomic analysis and isolation of RNA polymerase II dependent promoters from Spodoptera frugiperda. PLOS ONE 10(8):e0132898

 

PubMed entries of Maren

ReasearchGate Profile of Maren

ORCID (Original Citation ID)