Department Director
Room: 106
Phone: +49.531.391.5760
Fax: +49.531.391.5763
e-mail: m.hust@tu-braunschweig.de
Research interests
Antibody Engineering:
- recombinant antibodies
- antibody phage display
- antibody gene libraries
- antibodies for diagnostic and therapy with a focus on pathogens on toxins e.g. AntiBotABE, CDiff, DATMAB, HANTOX, ATAC (Antibody Therapy against Corona) and CORAT.
- CAR-T cells/ CAR-Tregs, e.g. geneTIGA
Biomarker and vaccines:
- identification of disease related biomarkers (immunogenic oligopeptides) using phage display
- biomarkers for diagnostic of pathogens
- develoment of an anti-tick vaccine (GO-Bio8 project) (Info Video)
Curriculum vitae
10/1993 - 7/1999 Studies of Biology at the Carl-von-Ossietzky Universität, Oldenburg.
4/1998 - 7/1999 Diploma thesis: "Molekularbiologische Untersuchungen zur Diversität von Diatomeenarten und Nachweis der Expressionsraten von Diatomeen fcp Genen im Wattsediment" (Molecularbiological analysis of diatom diversity and investigations on expression rates of the diatom fcp genes in the Wadden Sea sediments) in the AG Geomikrobiologie of the Institut für Chemie und Biologie des Meeres under the supervison of Prof. W.E. Krumbein and PD Dr. Erhard Rhiel.
9/1999 - 7/2002 PhD thesis: "Rekombinante Antikörper gegen die NIa-Protease des Plum Pox Virus" (Recombinant antibodies against the NIa protease of the plum pox virus) at the Lehrgebiet Molekulargenetik (now Institut für Pflanzengenetik, Abteilung Pflanzenbiotechnologie), Universität Hannover (now Leibniz Universität Hannover) under the supervision of Prof. Dr. H.-J. Jacobsen and Dr. Thomas Reinard.
12/2002 Group leader at the Institut für Biochemie und Biotechnologie, Abteilung Biotechnologie, Technische Universität Braunschweig headed by Prof. Dr. Stefan Dübel. Lecturer for molecular biotechnology.
5/2011 Habilitation (professorial dissertation, venia legendi for biotechnology), appointed as Privatdozent (PD).
12/2014 Appointed as professor for biotechnology (außerplanmäßiger Professor).
since 2022 Department Director, Medical Biotechnology
Publications
2024
(193) Metzdorf, K., Jacobsen, H., Kim, Y., Teixeira Alves, L.G., Kulkarni, U., Brdovčak, M.C., Materljan, J., Eschke, K., Chaudhry, M.Z., Hoffmann, M., Bertoglio, F., Ruschig, M., Hust, M., Šustić, M., Krmpotic, A., Jonjic, S., Widera, M., Ciesek, S., Pöhlmann, S., Landthaler, M. & Čičin-Šain, L. (2024). A single-dose MCMV-based vaccine elicits long-lasting immune protection in mice against distinct SARS-CoV-2 variants. Frontiers Immunology 15: 1383086
(192) Ruschig, M., Nerlich, J., Becker, M., Meier, D., Polten, S., Cervantes-Luevano, K., Kuhn, P., Licea Navarro, A.F., Hallermann S., Dübel, S., Schubert M., Brown, J. & Hust, M. (2024). Human antibodies neutralizing the alpha-latrotoxin of the European black widow. Frontiers Immunology 15: 1407398
(191) Marcotte, H., Cao, Y., Zuo, F., Simonelli, L., Sammartino, J.C., Pedoti, M., Sun, R., Cassaniti, I., Hagbom, M., Piralla, A., Yang, J., Du, L., Percivalle, E., Bertoglio, F., Schubert, M., Abolhassani, H., Sherina, N., Guerra, C., Borte, S., Rezaei, N., Kumagai-Braesch, M., Xue, Y., Su, C., Yan, Q., He, P., Grönwall, C., Klareskog, L., Calzolai, L., Cavalli, A., Wang, Q., Robbiani, D., Hust, M., Shi, Z., Feng, L., Svensson, L., Chen, L., Bao, L., Baldanti, F., Xiao, J., Qin, C., Hammarström, L., Yang, Y., Varani, L., Xie, X.S. & Pan-Hammarström, Q. (2024). Conversion of monoclonal IgG to dimeric and secretory IgA restores neutralizing ability and prevents infection of Omicron lineages. PNAS 121(3):e2315354120
(190) Zuo, F., Cao, Y., Sun, R., Yisimayi, A., Du, L., Bertoglio. F., Schubert, M., Guerra, C., Cavalli, A., Hust, M., Robbiani, D.F., Abolhassani, H., Xie, X.S., Hammarström, L., Marcotte, H. & Pan-Hammarström, Q. (2024). Neutralisation activity of mucosal IgA is more sustainable than serum IgG against XBB sublineages and BA.2.86. Lancet Infectious Diseases 24(19): e7-e9
2023
(189) Abassi, L., Bertoglio, F., Mačak-Šafranko, Ž., Schirrmann, T., Grewling-Pils, M., Seifert, O., Khan, F., Katzmarzyk, M., Jacobsen, H., Gödecke, N., Heine, P.A., Frenzel, A., Nowack, H., Dübel, S., Kurolt, I.-C., Kontermann, R.E., Markotić, A., Schubert, M., Hust, M. & Čičin-Šain, L. (2023). Evaluation of the neutralizing antibody STE90-C11 against SARS-CoV-2 Delta infection and its recognition of other variants of concern. Viruses 15: 2153
(188) Lutz, S., Klaus, K., Albici, A.-M., Ebinger, L., Sellmer, L., Teipel, H., Frenzel, A., Langner, A., Winterberg, D., Krohn, S., Hust, M., Schirrmann, T., Dübel, S., Scherließ, R., Humpe, A., Gramatzki, M., Kellner, C.& Peipp, M. (2023). Novel NKG2D-Directed Bispecific Antibodies Enhance Antibody-Mediated Killing of Malignant B Cells by NK Cells and T Cells. Frontiers Immunology 14: 1227572
(187) Dragon, A.C., Beermann, L.M., Umland, M., Bonifacius, A., Ruhl, L., Kehler, P., Gellert, J., Weiß, L., Mayer-Hain, S., Zimmermann, K., Riese, S., Thol, F., Beutel, G., Maecker-Kolhoff, B., Yamamoto, F., Blasczyk, R., Schambach, A., Hust, M., Hudecek, M. & Eiz-Vesper, B. (2023). CAR-Ts redirected against the Thomsen-Friedenreich antigen CD176 mediate specific elimination of malignant cells from leukemia and solid tumors. Frontiers Immunology 14: 1219165
(186) Steinke, S., Roth, K.D.R., Englick, R., Langreder, N., Ballmann, R., Fühner, V., Zilkens, K.J.K., Moreira, G.M.S.G., Koch, A., Russo, G., Schubert, M., Bertoglio, F., Heine, P.A. & Hust, M. (2023). Mapping Epitopes by Phage Display. Methods Molecular Biology 2702, 563-585
(185) Heine, P.A., Ballmann, R., Thevarajah, P., Russo, G., Moreira, G.M.S.G. & Hust, M. (2023). Biomarker Discovery by ORFeome Phage Display. Methods Molecular Biology 2702, 543-561
(184) Langreder, N., Schäckermann, D., Unkauf, T., Schubert, M., Frenzel, A., Bertoglio, F. & Hust, M. (2023). Antibody Affinity and Stability Maturation by Error-Prone PCR. Methods Molecular Biology 2702, 395-410
(183) Heine, P.A., Ruschig, M., Langreder, Wenzel, E.V., Schubert, M., Bertoglio, F. & Hust, M. (2023). Antibody Selection in Solution Using Magnetic Beads. Methods Molecular Biology 2702, 261-274
(182) Steinke, S., Roth, K.D.R., Ruschig, Langreder, N., Polten, S., Schneider, K.-T., Ballmann, R., Russo, G., Zilkens, K.J.K., Schubert, M., Bertoglio, F. & Hust, M. (2023). Antibody Selection via Phage Display in Microtiter Plates. Methods Molecular Biology 2702, 247-260
(181) Ruschig, M., Heine, P.A., Fühner, V., Zilkens, K.J.K., Steinke, S., Schubert, M., Bertoglio, F. & Hust, M. (2023). Construction of Human Immune and Naive scFv Phage Display Libraries. Methods Molecular Biology 2702, 15-37
(180) Nur, A., Schubert, M., Lai, J.Y., Hust, M., Choong, Y.S., Isa, W.Y.H.W. & Lim TS. (2023). Antibody Phage Display. Methods Molecular Biology 2702, 3-12
2022
2021
2020
2019
2018
(126) Burke, C.W., Froude, J.W., Miethe, S., Hülseweh, B., Hust, M. & Glass, P.J. (2018). Human-Like Neutralizing Antibodies Protect Mice from Aerosol Exposure with Western Equine Encephalitis Virus. Viruses 10: 147
(125) Sadana, P., Geyer, R., Pezoldt, J., Helmsing, S., Huehn, J., Hust, M., Dersch, P. & Scrima, A. (2018). The invasin D protein from Yersinia pseudotuberculosis selectively binds the Fab region of host antibodies and affects colonization of the intestine. Journal of Biological Chemistry 293: 8672-8690
2017
2016
2015
2014
2013
2012
2011
(57) Colwill, K., Persson, H., Jarvik, N.E., Wyrzucki, A., Wojcik, J., Koide, A., Kossiakoff, A.A., Koide, S., Sidhu, S., Dyson, M.R., Pershad, K., Pavlovic, J.D., Karatt-Vellat, A., Schofield, D.J., Kay, B.K., McCafferty, J., Mersmann, M., Meier, D., Mersmann, J., Helmsing, S., Hust, M., Dübel, S., Berkowicz, S., Freemantle, A., Spiegel, M., Sawyer, A., Layton, D., Nice, E., Dai, A., Rocks, O., Williton, K., Fellhouse, F.A., Hersi, K., Pawson, T., Nilsson, P., Sundberg, M., Sjöberg, R., Sivertsson, A., Schwenk, J.M., Takanen, J.O., Hober, S., Uhlén, M., Dahlgren, L.G., Flores, A. Johansson, I, Weigelt, J., Crombet, L., Loppnau, P., Kozieradzki, I., Cossar, D., Arrowsmith, C.H., Edwards, A.M. & Gräslund, S. (2011). A roadmap to generate renewable protein binders to the human proteome. Nature Methods 8, 551-558
2010
2009
2008
2007
2006
2005
(6) Hust, M. & Dübel, S. (2005). Phage display vectors for the in vitro generation of human antibody fragments. Immunochemical Protocols, 3rd ed., Ed: Burns, R., Methods Molecular Biology 295, 71-95
2004
(5) Hust, M. & Dübel, S. (2004). Mating antibody phage display with proteomics. Trends in Biotechnology 22, 8-14
2003
(4) Meyer, T., Hust, M., Marquardt, J., Krumbein, W.E. & Rhiel, E. (2003). A methodological approach to investigate steady state fucoxanthin chlorophyll a/c binding protein mRNA levels in Wadden Sea sediments. International Microbiology 6, 33-39
2002
(3) Hust, M., Maiss, E., Jacobsen, H.-J. & Reinard, T. (2002). The production of a genus specific recombinant antibody (scFv) using a recombinant Potyvirus protease. Journal of Virological Methods 106, 225-233
1999
(2) Hust, M., Krumbein, W.E. & Rhiel, E. (1999). An immunochemical in situ approach to detect adaptation processes in the photosynthetic apparatus of diatoms of the Wadden Sea sediment surface layers. Journal of Microbiological Methods 38, 69-80
(1) Janssen, M., Hust, M., Rhiel, E. & Krumbein, W.E. (1999). Vertical migration behaviour of diatom assemblages of Wadden Sea sediments (Dangast/Germany): A study using Cryo-scanning electron microscopy. International Microbiology 2, 103-110
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