Oostlander AG, Brodde L, von Bargen M, Slippers B, Becker Y, Brandt U, et al. (2024) Development of a molecular genetics and cell biology toolbox for the filamentous fungus Diplodia sapinea. PLoS ONE 19(12): e0308794. doi.org/10.1371/journal.pone.0308794
You, Y.; Suraj, H.M.; Matz, L.; Herrera Valderrama, A.L.; Ruigrok, P.; Shi-Kunne, X; Pieterse, F.P.J.; Oostlander, A.G; Beenen, H.G.; Chavarro-Carrero, E.A.; Qin, S.; Verstappen, F.W.A.; Kappers, I.F.; Fleißner, A.; van Kan, J.A.L.: (2024) Botrytis cinerea combines four molecular strategies to tolerate membrane-permeating plant compounds and to increase virulence. In: Nature Communications 15 (1), S. 6448.
DOI: 10.1038/s41467-024-50748-5.
Oostlander, A.G., Brodde, L., von Bargen, M., Leiterholt, M., Trautmann, D., Enderle, R., Elfstrand, M., Stenlid, J, and Fleißner, A. (2023) A reliable and simple method for the production of viable pycnidiospores of the pine pathogen Diplodia sapinea and a spore-based infection assay on Scots pine. Plant Disease doi.org/10.1094/PDIS-01-23-0107-RE.
Fleißner, A., Oostlander, A., and Well, L. (2022) Highly conserved, but highly specific: somatic cell-cell fusion in filamentous fungi. Current Opinion in Cell Biology, doi: 10.1016/j.ceb.2022.102140.
Hammadeh, H.H., Serrano, A., Wernet, V., Stomberg, N., Hellmeier, D., Weichert, M., Brandt, U., Sieg, B., Kanofsky, K., Hehl, R., Fischer, R., and Fleißner, A. (2022) A dialog-like cell communication mechanism is conserved in filamentous ascomycete fungi and mediates interspecies interactions. Proc. Natl. Acad. Sci. USA, doi.org/10.1073/pnas.2112518119.
Gabriel, R., Mueller, R., Floerl, L., Hopson, C., Harth, S., Schuerg, T., Fleißner, A., and Singer, S. (2021) CAZymes from the thermophilic fungus Thermoascus aurantiacus are induced by C5 and C6 sugars. Biotechnology for Biofuels, 14(1):169. doi: 10.1186/s13068-021-02018-5
Gabriel, R., Thieme, N., Liu, Q., Li, F., Kohler, L.T., Harth, S.m Jecmenica, M., Ramamurthy, M., Gorman, J., Simmons, B., McCluskey, K., Baker, S.E., Tian, C., Schürg, T., Singer, S.W., Fleißner, A.#, and Benz, J.P.# (2021) The F-box protein gene exo-1 is a new target for reverse-engineering enzyme hypersecretion in filamentous fungi. Proc. Natl. Acad. Sci. USA.118(26):e2025689118. doi: 10.1073/pnas.2025689118.
Weichert, M., Herzog, S., Robson, S.A., Brandt, R., Priegnitz, B.E., Brandt, U., Schulz, S., and Fleißner, A. (2020) Plasma membrane fusion is specifically impacted by the molecular structure of membrane sterols during vegetative development of Neurospora crassa. Genetics, 216(4):1103-1116.
Gabriel, R., Jecmenica, M., Prinz, J., Floerl, L., Romero-Vasquez, C., Oostlander, A., Matz, L., Harth, S., Leynaud-Kieffer, L., Fritsche, S., Gorman, J., Hopson, C., Schuerg, T., Fleissner, A., and Singer, S.W. (2020) Development of genetic tools for the thermophilic filamentous fungus Thermoascus aurantiacus. Biotechnology for Biofuels, doi: 10.1186/s13068-020-01804-x.
Tanaka, A., Kamiya, S., Ozaki, Y., Kameoka, S., Kayano, Y., Sanjay, S., Akano, F., Uemura, A., Takagi, H., Terauchi, R., Maruyama, J., Hammadeh, H.H., Fleißner, A., Scott, B., and Takemoto, D. (2020) A nuclear protein NsiA from Epichloë festucae interacts with a MAP kinase MpkB and regulates the expression of genes required for symbiotic infection and hyphal cell fusion. Molecular Microbiology 114(4):626-640.
Herzog, S., Brinkmann, H., Vences, M., and Fleißner, A. (2020) Evidence of repeated horizontal transfer of sterol C-5 desaturase encoding genes among dikarya fungi. Molecular Phylogenetics and Evolution 150:106850.
Petersen, I., Herzog, S., Bath, C., and Fleißner, A. (2020) Contextualisation of factual knowledge in genetics: A pre- and post-survey of undergraduates´understanding of the Nature of Science. Interdisciplinary Journal of Environmental and Science Education 16(2), e2215.
Hassing, B., Eaton, C.J., Winter, D., Green, K.A., Brandt, U., Savoian, M.S., Mesarich, C.H., Fleißner, A., and Scott, B. (2020) Phosphatidic acid produced by phospholipase D is required for hyphal cell-cell fusion and fungal-plant symbiosis. Molecular Microbiology 113(6):1101-1121.
Schumann, M.R., Brandt, U., Adis, C., Hartung, L., and Fleißner, A. (2019) Plasma membran integrity during cell-cell fusion and in response to pore-forming drugs is promoted by the penta-EF-hand protein PEF1 in Neurospora crassa. Genetics 213(1):195-211.
Serrano, A., Illgen, J., Brandt, U., Thieme, N., Letz, A., Lichius, A., Read, N.D., and Fleißner, A. (2018) Spatio-temporal MAP kinase dynamics mediate cell behavior coordination during fungal somatic cell fusion. Journal of Cell Science 131(9): jcs213462.
Havlik, D., Brandt, U., Bohle, K., and Fleißner, A. (2017) Establishment of Neurospora crassa as a host for heterologous protein production using a human antibody fragment as a model product. Microbial Cell Factories 16(1):128. doi: 10.1186/s12934-017-0734-5.
Serrano, A., Hammadeh, H., Herzog, S., Illgen, J., Schumann, M.R., Weichert, M., and Fleißner, A. (2017) The dynamics of signal complex formation mediating germling fusion in Neurospora crassa. Fungal Genetics and Biology 101:31-33.
Weichert, M., Lichius, A., Priegnitz, B.E., Brandt, U., Gottschalk, J., Nawrath, T., Groenhagen, U., Read, N.D., Schulz, S., and Fleißner A. (2016) Accumulation of specific sterol precursors targets a MAP kinase cascade mediating cell-cell recognition and fusion. Proc. Natl. Acad. Sci. USA. 113(42): 11877-11882.
Daskalov, A., Heller, J., Herzog, S., Fleißner, A., and Glass, N.L. (2016) Molecular mechanisms regulating cell fusion and heterokaryon formation in filamentous fungi. In Heitman, J., Howlett, B., Crous, P., Stukenbrock, E., James, T., and Gow, N. (eds) The Fungal Kingdom, in press
Fleißner, A., and Herzog, S. (2016) Signal exchange and integration during self-fusion in filamentous fungi. Seminars in Cell and Developmental Biology, 57:76-83.
Heine, D., Petereit, L., Schumann, M.R., Patzelt, D., Rachid, L., Brandt, U., Werner, A., Pöggeler, S., and Fleißner, A. (2016) The tetraspanin TSP3 of Neurospora crassa is a vacuolar membrane protein and shares characteristics with IDI proteins. Mycologia, 108(3): 581-589.
Herzog, S., Schumann, M.R., and Fleißner, A. (2015) Cell fusion in Neurospora crassa. Current Opinion in Microbiology, 28: 53-59.
Fleißner, A., and Serrano, A. (2015) The art of networking: Vegetative hyphal fusion in filamentous ascomycete fungi. In Wendland, J. (ed.) The Mycota Vol. 1, 133-153.
Priegnitz, B.E., Brandt, U., Pahirulzaman, K.A.K., Dickschat, J.S., and Fleißner, A. (2015) The AngFus3 MAP kinase controls hyphal differentiation and secondary metabolism in Aspergillus niger. Eukaryotic Cell, 14(6): 602-615.
Weichert, M., and Fleißner, A. (2015) Anastomosis and Heterokaryon Formation. In Van den Berg, M. and Maruthachalam, K. (eds.) Genetic Transformation Systems in Fungi Vol. 2: 3-21.
Wagner, D., Wiemann, P., Huß, K., Brandt, U., Fleißner, A., and Tudzynski, B. (2013) A sensing role of the glutamine synthetase in the nitrogen regulation network in Fusarium fujikuroi. PLoS ONE, DOI: 10.1371/journal.pone.0080740.
Dettmann, A., Heilig, Y., Ludwig, S., Schmitt, K., Illgen, J., Fleißner, A., Valerius, O., Seiler, S. (2013) HAM-2 and HAM-3 are central for the assembly of the Neurospora STRIPAK complex at the nuclear envelope and regulate nuclear accumulation of the MAP kinase MAK-1 in a MAK-2-dependent manner. Molecular Microbiology, 90(4): 796-812.
Weichert, M. und Fleißner, A. (2013) Zellen im Dialog: Zellfusionen im roten Brotschimmel Neurospora crassa. BIOspektrum 10.1007/s12268-013-0323-z
Aguilar, P.S., Baylies, M.K., Fleißner, A., Helming, L., Inoue, N., Podbilewicz, B., Wang, H., and Wong, M. (2013) Genetic basis of cell-cell fusion mechanisms. Trends in Genetics, 29(7): 427-437.
Fleißner, A. (2013) Turning the switch: Using chemical genetics to elucidate protein kinase functions in filamentous fungi. Fungal Biology Reviews, 27(1): 25-31.
Schürg, T., Brandt, U., Adis, C., and Fleißner, A. (2012) The Saccharomyces cerevisiae BEM1 homolog in Neurospora crassa promotes coordinated cell behavior resulting in cell fusion. Molecular Microbiology, 86: 349-366.
Dettmann, A., Illgen, J., März, S., Schürg, T., Fleißner, A., and Seiler, S. (2012) The NDR kinase scaffold HYM1/MO25 is essential for MAK2 MAP kinase signaling in Neurospora crassa. PLoS Genetics DOI: 10.1371/journal.pgen.1002950.
Roca, M.G., Weichert, M., Siegmund, U., Tudzynski, P., and Fleißner, A. (2012) Germling fusion via conidial anastomosis tubes in the gray mold Botrytis cinerea requires NADPH oxidase activity. Fungal Biology, 116(3): 379-387.
Priegnitz, B.E., Wargenau, A., Brandt, U., Rohde, M., Dietrich, S., Kwade, A., Krull, R., and Fleißner, A. (2012) The role of initial spore adhesion in pellet and biofilm formation in Aspergillus niger. Fungal Genetics and Biology, 49(1): 30-38.
Fleißner, A. (2012) Hyphal fusion. In Perez-Martin, J., and Di Pietro, A. (eds.) Morphogenesis and pathogenicity in fungi. Topics in Current Genetics Springer-Verlag. Vol. 22 pp 43-60.
Wargenau, A., Fleißner, A., Bolten, C.J., Rohde, M., Kampen, I., and Kwade, A. (2011) On the origin of the electrostatic surface potential of Aspergillus niger spores in acidic environments. Research in Microbiology. 162(10): 1011-1017.
Riquelme, M., Yarden, O., Bartnicki-Garcia, S., Bowman, B., Castro-Longoria, E., Free, S.J., Fleißner, A., Freitag, M., Lew, R.R., Mouriño-Pérez, R., Plamann, M., Rasmussen, C., Richthammer, C., Roberson, R.W., Sánchez-León, E., Seiler, S., Watters, M.K. (2011) Architecture and development of the Neurospora crassa hypha - a model cell for polarized growth. Fungal Biology 115(6): 446-474.
Fleißner, A., and Dersch P. (2010) Expression and Export: Recombinant protein production systems for Aspergillus. Applied Microbiology and Biotechnology. 87(4): 1255-1270.
Read, N. D., Fleißner, A., Roca, M. G. and Glass, N. L. (2010) Hyphal fusion. In Cellular and Molecular Biology of Filamentous fungi, pp 260-273. ASM press.
Fleißner, A., Leeder, A.C., Roca, M.C., Read, N.D., and Glass, N.L. (2009) Oscillatory recruitment of signaling proteins to cell tips promotes coordinated behavior during cell fusion. Proc. Natl. Acad. Sci. USA. 106(46):19387-19392.
Fleißner, A., Diamond, S. and Glass, N.L. (2009) The Saccharomyces cerevisiae PRM1 homolog in Neurospora crassa is involved in vegetative and sexual cell fusion events, but also has postfertilization functions. Genetics 181(2): 497-510.
Fleißner, A., Simonin, A.R. and Glass, N.L. (2008) Cell fusion in the filamentous fungus Neurospora crassa. Methods in Molecular Biology 475: 21-38.
Fleißner, A. and Glass, N.L. (2007) SO, a protein involved in hyphal fusion in Neurospora crassa, localizes to septal plugs. Eukaryotic Cell 6(1): 84-94.
Glass, N.L. and Fleißner, A. (2006) Re-wiring the network: understanding the mechanism and function of hyphal fusion in filamentous fungi. In Kües, U. and Fischer, R. (eds.) The Mycota Vol. 1: 123-139.
Fleißner, A., Sarkar, S., Jacobson, D.J., Roca, M.G., Read, N.D. and Glass, N.L. (2005) The so locus is required for vegetative cell fusion and postfertilization events in Neurospora crassa. Eukaryotic Cell 4(5): 920-930.
Fleißner, A., Sopalla, C. and Weltring, K.-M. (2002) An ATP-binding multidrug resistance transporter is necessary for tolerance of Gibberella pulicaris to phytoalexins and virulence on potato tubers. Molecular Plant Microbe Interactions 15(2): 102-108.