Publikationen

2025

PUBMED: 40013841
Schilling, T., Biedendieck, R., Moran-Torres, R., Saaranen, M.J., Ruddock, L.W., Daniel, R. & van Dijl, J.M. (2025)
Towards Antibody Production in Genome-Minimized Bacillus subtilis Strains.
ACS SynBio. in press

2024

DOI: 10.1038/s41598-024-78535-8
Zangenehzadeh, S., Agocs, E., Schröder, F., Amroun, N., Biedendieck, R., Jahn, D., Günther, A., Zheng, L., Roth, B., Johannes, H.-H. & Kowalsky, W. (2024)
Polarization modulated spectroscopic ellipsometry-based surface plasmon resonance biosensor for E. coli K12 detection.
Sci Rep. 14, 27046

DOI: 10.1016/j.procir.2024.08.204
Zimbelmann, S., Hintmann, M., Baumann, M., Jahn, D., Biedendieck, R. & Emde, B. (2024)
Selective Lethal Impact of Blue Laser Radiation Compared to High-Power LED Irradiation on Naturally Grown Biofouling in the Context of Underwater Biofouling Cleaning.
Procedia CIRP. 124, 696–699

DOI: 10.1007/978-3-031-63164-1_1
Wichmann, J., Staar, M., Kubiak, M., Solarczek, J., Mayer, J., Jahn, D., Kampen, I., Schilde, C., Schallmey, A. & Biedendieck, R. (2024)
Correlation of structure and properties of enzyme crystals in consideration of downstream processing and formulation.
In "Dispersity, Structure and Phase Changes of Proteins and Bio Agglomerates in Biotechnological Processes", Kwade, A. and Kampen, I. (Eds.) Springer Nature, S. 1-57

DOI: 10.1007/978-3-031-63164-1_6
Brune, S.E., Jahn, D., Krull, R. & Biedendieck, R. (2024)
Production, purification, and characterization of recombinant β-lactoglobulin variants.
In "Dispersity, Structure and Phase Changes of Proteins and Bio Agglomerates in Biotechnological Processes", Kwade, A. and Kampen, I. (Eds.) Springer Nature, S. 181-200

DOI: 10.1007/s11947-024-03414-z
Hoppenreijs, L.J.G., Brune, S.E., Biedendieck, R., Krull, R., Boom, R.M. & Keppler, J.K. (2024)
Exploring functionality gain for (recombinant) β-lactoglobulin through production and processing interventions.
Food Bioproc Tech.

PUBMED: 38544863
Mingers, T., Mass, V., Barthels, S., Borrero De Acuña, S.M., Biedendieck, R., Cooke, A., Dailey, T., Gerdes, S., Blankenfeld, W., Dailey, H., Warren, M., Jahn, M. & Jahn, D. (2024)
The Alternative Coproporphyrinogen III Oxidase (CgoN) Catalyzes the Oxygen-independent Conversion of Coproporphyrinogen III into Coproporphyrin III.
Front Microbiol. 15, 1378989

DOI: 10.3390/photonics11030220
Hintmann, M., Zimbelmann, S., Emde, B., Biedendieck, R. & Jahn, D. (2024)
Antibiotic Effect of High-Power Blue Laser Radiation
Photonics 11, 220

2023

DOI: 10.1016/j.idairyj.2023.105772
Brune, S.E., Hoppenreijse, L.J.G., Kühl, T., Lautenbach, V., Walter, J., Peukert, W., Schwarz, K., Imhoff, D., Boom, R.M. Krull, R., Keppler, J.K. & Biedendieck, R. (2023)
Precision fermentation as a route to modify β-lactoglobulin structure through substitution of specific cysteine residues.
Int. Dairy J. 147, 105772

PUBMED: 37532778
Weber, J.-N., Minner-Meinen, R., Behnecke, M., Biedendieck, R., Hänsch, V., Hercher, T., van den Hout, L., Knüppel, L., Sivov, S., Schulze, J., Mendel, R.-R., Hänsch, R. & Kaufholdt, D. (2023)
Molybdate transport under the moon light: How the novel AtMOT2 family and GSH-complex formation close gaps in molybdenum homeostasis.
Commun Biol. 6, 801

PUBMED: 37187417
Hoppenreijs, L.J.G., Overbeck, A., Brune, S.E., Biedendieck, R., Kwade, A., Krull, R. Boom, R.M. & Keppler, J.K. (2023)
Amyloid-like aggregation of recombinant β-lactoglobulin at pH 3.5 and 7.0: is disulfide bond removal the key to fibrillation?
Int J Biol Macromol. 242, 124855

DOI: 10.1016/j.foodhyd.2023.108727
Hoppenreijs, L.J.G., Brune, S.E., Biedendieck, R., Krull, R., Boom, R.M. & Keppler, J.K. (2023)
Fibrillation of β-lactoglobulin at pH 2.0: impact of cysteine substitution and disulfide bond reduction
Food Hydrocoll. 141, 108727

DOI: 10.1021/acs.cgd.2c01408
Wichmann, J., Mayer, J., Hintmann, M., Lukat, P., Blankenfeldt, W. & Biedendieck, R. (2023)
Multi-step engineering of a penicillin G acylase for systematic improvement of crystallization efficiency
Cryst Growth Des. 23, 3230–3243

DOI: 10.1016/j.tsf.2022.139583
Zangenehzadeh, S., Agocs, E., Jivani, H., Könemund, L., Neumann, L., Hirschberg, F., Herdan, S., Biedendieck, R., Jahn, D., Roth, B.W., Johannes, H.-H. & Kowalsky, W. (2023)
Bacteria detection in a Kretschmann geometry flow cell at a plasmon-enhanced interface with spectroscopic ellipsometer.
Thin Solid Films., 764, 139583

2019

PUBMED: 31649093
Thiel, S., Bunk, B., Spröer, C., Overmann, J., Jahn, D. & Biedendieck, R. (2019)
Complete genome sequence of Raoultella electrica 1GB (DSM 102253T), isolated from anodic biofilms of a glucose-fed microbial fuel cell.
Microbiol Resour Announc 8. pii: e00800-19

PUBMED: 31616807
Kleinfeldt, L., Gädke, J., Biedendieck, R., Krull, R. & Garnweitner, G. (2019)
Spray-dried hierarchical aggregates of iron oxide nanoparticles and their functionalization for downstream processing in biotechnology.
ACS Omega 4:16300-16308

PUBMED:  31227867
Mayer, J., Pippel, J., Günther, G., Müller, C., Lauermann, A., Knuuti, T., Blankenfeldt, W., Jahn, D. & Biedendieck, R. (2019)
Crystal structures and protein engineering of three different penicillin G acylases from Gram-positive bacteria with different thermostability.
Appl Microbiol Biotechnol. 103:7537-7552

PUBMED: 30835726
Münch, K., Münch, R., Biedendieck, R., Jahn, D. & Müller, J. (2019)
Evolutionary model for the unequal segregation of high copy plasmids.
PLOS Comp Biol. 15:e1006724

2018

PUBMED: 30186274
Hofmann, J. D., Otto, A., Berges, M., Biedendieck, R., Michel, A.-M., Becher, D., Jahn, D. & Neumann-Schaal, M. (2018)
Metabolic reprogramming of Clostridioides difficile during the stationary phase with the induction of toxin production.
Front Microbiol. 9:1970

PUBMED: 29666238
Moore, S.J., MacDonald, J.T., Wienecke, S., Ishwarbhai, A., Tsipa, A., Aw, R., Kylilis, N., Bell, D.J., McClymont, D.W., Jensen, K., Polizzi, K.M., Biedendieck, R. & Freemont, P.S. (2018)
Rapid acquisition and model-based analysis of cell-free transcription-translation reactions from non-model bacteria
Proc Natl Acad Sci USA. 115:E4340-E4349

PUBMED: 28917535
Gädke, J., Thies, J.W., Kleinfeldt, L., Schulze, T., Biedendieck, R., Rustenbeck, I., Garnweitner, G., Krull, R. & Dietzel, A. (2018)
Selective manipulation of superparamagnetic nanoparticles for product purification and microfluidic diagnostics.
Eur J Pharm Biopharm. 126:67-74

PUBMED: 28606596
Lakowitz, A., Godard, T., Biedendieck, R. & Krull, R. (2018)
Mini review: Recombinant production of tailored bio-pharmaceuticals in different Bacillus strains and future perspectives
Eur J Pharm Biopharm. 126:27-39

2017

DOI
Gädke, J., Thies, J.-W., Kleinfeldt, L., Kalinin, A., Starke, G., Lakowitz, A., Biedendieck, R., Garnweitner, G., Dietzel, A. & Krull, R. (2017)
Integrated in situ-purification of recombinant proteins from Bacillus megaterium cultivations using SPION in stirred tank reactors.
Biochem Eng J. 126: 58-67

PUBMED: 29086039
Blaženović, I., Kind, T., Torbašinović, H., Obrenović, S., Mehta, S.S., Tsugawa, H., Wermuth, T., Schauer, N., Jahn, M., Biedendieck, R., Jahn, D. & Fiehn, O. (2017)
Comprehensive comparison of in silico MS/MS fragmentation tools of the CASMI contest: database boosting is needed to achieve 93% accuracy
J Cheminform. 9:32

PUBMED: 28115011
Lakowitz, A., Krull, R. & Biedendieck, R. (2017)
Recombinant production of the antibody fragment D1.3 scFv with different Bacillus strains.
Microb Cell Fact. 16: 14

PUBMED: 27888121
Gädke, J., Kleinfeldt, L., Schubert, C., Rohde, M., Biedendieck, R., Garnweitner, G. & Krull, R. (2017)
In situ affinity purification of His-tagged protein A from Bacillus megaterium cultivation using recyclable superparamagnetic iron oxide nanoparticles.
J Biotechnol. 242: 55-63

2016

PUBMED: 26853478
Ferrer, A., Bunk, B., Spröer, C., Biedendieck, R., Valdés, N., Jahn, M., Jahn, D., Orellana, O. & Levican, G. (2016)
Complete genome sequence of the bioleaching bacterium Leptospirillum sp. group II strain CF-1.
J Biotechnol. 222: 21-22.

PUBMED: 27165321
Biedendieck, R. (2016)
A Bacillus megaterium system for the production of recombinant proteins and protein complexes - in "Advances in Experimental Medicine and Biology / Advanced Technologies for Protein Complex Production" (Vega, C., Ed.)
Springer, Berlin, Heidelberg pp. 97-113

2015

PUBMED: 26215140
Gerber, A., Kleser, M., Biedendieck, R., Bernhardt, R. & Hannemann, F. (2015)
Functionalized PHB granules provide the basis for the efficient side-chain cleavage of cholesterol and analogs in recombinant Bacillus megaterium.
Microb Cell Fact. 14:107

PUBMED: 26116677
Münch, K., Müller, J., Wienecke, S., Bergmann, S., Heyber, S., Biedendieck, R., Münch, R. & Jahn, D. (2015)
Polar fixation of plasmids during recombinant protein production in Bacillus megaterium results in population heterogeneity.
Appl Environ Microbiol. 81:5976-5986

PUBMED: 26138251
Finger, C., Gamer, M., Klunkelfuß, S., Bunk, B. & Biedendieck, R. (2015)
Impact of rare codons and the functional coproduction of rate-limiting tRNAs on recombinant protein production in Bacillus megaterium
Appl Microbiol Biotechnol. 99:8999-9010.

2014

Masthoff, I., Gädke, J., Krull, R., Biedendieck, R. & Garnweitner (2014)
Funktionalisierte Nanopartikel - Biokompatible Eisenoxid-Nanopartikel zur in situ-Magnetseparation von Bioprodukten
CITplus 10:46-48.

Schulz, A., Fräger, M., Holtkamp, L., Ronnenberg, J. & Biedendieck, R. (2014)
Bacillus megaterium - ein Produktionssystem für rekombinante Proteine.
BIOspektrum 06:650-651.

PUBMED: 24657453
Moore, S.J., Mayer, M.J., Biedendieck, R., Deery, E. & Warren, M. (2014)
Towards a cell factory for vitamin B12 production in Bacillus megaterium: Bypassing of the cobalamin riboswitch control elements.
N Biotechnol. 31:553-561.

2013

PUBMED: 23922391
Moore, S.J., Lawrence, A.D., Biedendieck, R., Deery, E., Frank, S., Howard, M.J., Rigby, S.E.J. & Warren, M.J. (2013)
Elucidation of the anaerobic pathway for the corrin component of cobalamin (vitamin B12).
Proc Natl Acad Sci USA 110: 14906-149011.

PUBMED: 23457476
Collins, H.F., Biedendieck, R., Leech, H.K., Gray, M., Escalante-Semerena, J.C., McLean, K.J., Munro, A.W., Rigby, S.E., Warren, M.J. & Lawrence, A.D. (2013)
Bacillus megaterium has both a functional BluB protein required for DMB synthesis and a related flavoprotein that forms a stable radical species.
PLoS One 8: e55708.

PUBMED: 23447448
Buschke, N., Becker, J., Schäfer, R., Kiefer, P., Biedendieck, R. & Wittmann, C. (2013)
Systems metabolic engineering of xylose utilizing Corynebacterium glutamicum for production of 1,5-diaminopentane.
Biotechnol J. 8: 557-570.

PUBMED: 22750448
Korneli, C., David, F., Biedendieck, R., Jahn, D. & Wittmann, C. (2013)
Getting the big beast to work--systems biotechnology of Bacillus megaterium for novel high-value proteins
J Biotechnol. 163: 87-96.

PUBMED: 23155054
Moore, S.J., Biedendieck, R., Lawrence, A.D., Deery, E., Howard, M.J., Rigby, S.E.J. & Warren, M.J. (2013)
Characterization of the enzyme CbiH60 involved in anaerobic ring contraction of the cobalamin (vitamin B12) biosynthetic pathway.
J Biol Chem. 288: 297-305.

PUBMED: 23179620
Korneli, C., Biedendieck, R., David, F., Jahn, D. & Wittmann, C. (2013)
High yield production of extracellular recombinant levansucrase by Bacillus megaterium.
Appl Microbiol Biotechnol. 97: 3343-3353.

2012

PUBMED: 22772947
Klein, J., Leupold, S., Biegler, I., Biedendieck, R., Münch, R. & Jahn, D. (2012)
TLM-Tracker: Software for cell segmentation, tracking and lineage analysis in time-lapse microscopy movies.
Bioinformatics 28: 2276-2277.

2011

PUBMED: 21935588
Borgmeier, C., Biedendieck, R., Hoffmann, K., Jahn, D. & Meinhardt, F. (2011)
Transcriptome profiling of degU expression reveals unexpected regulatory patterns in Bacillus megaterium and discloses new targets for optimizing expression.
Appl Microbiol Biotechnol. 92: 583-596.

PUBMED: 21705586
Eppinger, M., Bunk, B., Johns, M.A., Edirisinghe, J.N., Kutumbaka, K.K., Koenig, S.S.K., Creasy, H.H., Rosovitz, M.J., Riley, D.R., Daugherty, S., Martin, M., Elbourne, L.D.H., Paulsen, I., Biedendieck, R., Braun, C., Grayburn, Dhingra, S., Lukyanchuk, V., Ball, B., ul-Qamar, R., Seibel, J., Bremer, E., Jahn, D., Ravel, J. & Vary, P.S. (2011)
Genome sequences of the biotechnologically important B. megaterium strains QM B1551 and DSM319.
J Bacteriol. 193: 4199-4213.

PUBMED: 21454585
Strube, C.P., Homann, A., Gamer, M., Jahn, D., Seibel, J. & Heinz, D.W. (2011)
Polysaccharide synthesis of the levansucrase SACB from Bacillus megaterium is controlled by distinct surface motifs.
J Biol Chem. 286: 17593-17600.

PUBMED: 21943898
Biedendieck, R., Borgmeier, C., Bunk, B., Stammen, S., Scherling, C., Meinhardt, F., Wittmann, C. & Jahn, D. (2011)
Systems biology of recombinant protein production using Bacillus megaterium.
Methods Enzymol. 500: 165-195.

2010

PUBMED: 20596705
Stammen, S., Schuller, F., Dietrich, S., Gamer, M., Biedendieck, R. & Jahn, D. (2010)
Application of Escherichia coli phage K1E DNA-dependent RNA polymerase for in vitro RNA synthesis and in vivo protein production in Bacillus megaterium.
Appl Microbiol Biotechnol. 88: 529-539.

PUBMED: 20435764
Stammen, S., Müller, B.K., Korneli, C., Biedendieck, R., Gamer, M., Franco-Lara, E. & Jahn, D. (2010)
High yield intra- and extracellular protein production using Bacillus megaterium.
Appl Environ Microbiol. 76: 4037-4046.

PUBMED: 21326933
Bunk, B., Schulz, A., Stammen, S., Münch, R., Warren, M. J., Rohde, M., Jahn, D. & Biedendieck, R. (2010)
A short story about a big magic bug.
Bioeng Bugs 1: 1-7.

PUBMED: 21255303
Biedendieck, R., Malten, M., Barg, H., Bunk, B., Martens, J.-H., Deery, E., Leech, H., Warren, M. J. & Jahn, D. (2010)
Metabolic engineering of cobalamin (vitamin B₁₂) production in Bacillus megaterium.
Microb Biotechnol. 3: 24-37.

Bunk, B., Biedendieck, R., Jahn, D. & Vary, P. S. (2010)
Bacillus megaterium and other Bacilli, Industrial Applications
In Flickinger M. C. (Ed.) Encyclopedia of Industrial Biotechnology: Bioprocess, Bioseparation, and Cell Technology.
Volume 1 pp 429-443. John Wiley & Sons. Inc., Hoboken, NJ.

PUBMED: 20484676
Möbius, K., Arias-Cartin, R., Breckau, D., Haennig, A.-L., Riedmann, K., Biedendieck, R., Schroeder, S., Becher, D., Magalon, A., Moser, J., Jahn, M. & Jahn, D. (2010)
Heme biosynthesis is coupled to electron transport chains for energy generation
Proc. Natl. Acad. Sci. USA 107:10436-10441.

PUBMED: 20140656
Biedendieck, R., Bunk, B., Fürch, T., Franco-Lara, E., Jahn, M. & Jahn, D. (2010)
Systems Biology of Recombinant Protein Production in Bacillus megaterium - in "Advances in Biochemical Engineering / Biotechnology"
(Scheper, T., Ed.); Vol. 113 - Biosystems Engineering (Wittmann, C., Ed.)
Springer, Berlin, Heidelberg 120: 133-161.

2009

PUBMED: 19068481
Schroeder, S., Lawrence, A.D., Biedendieck, R., Rose, R.S., Deery, E., Graham, R.M., McLean, K.J., Munro, A.W., Rigby, S.E. & Warren, M.J. (2009)
Demonstration that CobG, the monooxygenase associated with the ring contraction process of the aerobic cobalamin (vitamin B12) biosynthetic pathway, contains an Fe-S center and a mononuclear non-heme iron center.
J Biol Chem 284: 4796-4805.

DOI
Beine, R., Valente, A.R., Biedendieck, R., Jahn, D. & Seibel, J. (2009)
Directed optimization of biocatalytic transglycosylation processes by the integration of genetic algorithms and fermentative approaches into a kinetic model.
Proc Biochem. 44: 1103-1114.

PUBMED: 19308404
Gamer, M., Froede, D., Biedendieck, R., Stammen, S., Jahn, D. (2009)
A T7 RNA polymerase-dependent gene expression system for Bacillus megaterium.
Appl Microbiol Biotechnol. 82: 1195-203.

2007

PUBMED: 17692983
Biedendieck, R., Gamer, M., Jaensch, L., Meyer, S., Rohde, M., Deckwer, W.-D. & Jahn, D. (2007)
A sucrose-inducible promoter system for the intra- and extracellular protein production in Bacillus megaterium.
J Biotechnol. 132: 426-430.

PUBMED: 17608626
Homann, A., Biedendieck, R., Goetze, S., Jahn, D. & Seibel, J. (2007)
Insights into polymer versus oligosaccharide synthesis - Mutagenesis and mechanistic studies of a novel levansucrase from Bacillus megaterium.
Biochem J. 407:189-198.

PUBMED: 17619232
Bäumchen, C., Roth, A. H. F. J., Biedendieck, R., Malten, M., Follmann, M., Sahm, H., Bringer-Meyer, S. & Jahn, D. (2007)
D-Mannitol production by resting state whole cell biotransformation of D-fructose by heterologous mannitol and formate dehydrogenase genes expression in Bacillus megaterium.
Biotechnol J. 2:1408-1416

PUBMED: 17657486
Vary, P. S., Biedendieck, R., Fuerch, T., Meinhardt, F., Rohde, M., Deckwer, W.-D. & Jahn, D. (2007)
Bacillus megaterium - from simple soil bacterium to industrial protein production host.
Appl Microbiol Biotechnol. 76:957-967. Review.

PUBMED: 17224052
Jordan, E., Hust, M., Roth, A., Biedendieck, R., Schirrmann, T., Jahn, D. & Duebel, S. (2007)
Production of recombinant antibody fragments in Bacillus megaterium
Microb Cell Fact. 6:2.

PUBMED: 17245578
Biedendieck, R., Beine, R., Gamer, M., Jordan, E., Buchholz, K., Seibel, J., Dijkhuizen, L., Malten, M. & Jahn, D. (2007)
Export, purification and activities of affinity tagged Lactobacillus reuteri levansucrase produced by Bacillus megaterium.
Appl Microbiol Biotechnol. 74:1062-1073.

PUBMED: 16964623
Biedendieck, R., Yang, Y., Deckwer, W.-D., Malten, M. & Jahn, D. (2007)
Plasmid system for the intracellular production and purification of affinity-tagged proteins in Bacillus megaterium.
Biotechnol Bioeng. 96:525-537.

2006

PUBMED: 17132166
Yang, Y., Biedendieck, R., Wang, W., Gamer, M., Malten, M., Jahn, D. & Deckwer, W.-D. (2006)
High yield recombinant penicillin G amidase production and export into the growth medium using Bacillus megaterium.
Microb Cell Fact. 5:36.

PUBMED: 16461726
Malten, M., Biedendieck, R., Gamer, M., Drews, A.C., Stammen, S., Buchholz, K., Dijkhuizen, L. & Jahn D (2006)
A Bacillus megaterium plasmid system for the production, export, and one-step purification of affinity-tagged heterologous levansucrase from growth medium.
Appl Environ Microbiol. 72:1677-1679.

Hollmann, R., Malten, M., Biedendieck, R., Yang, Y., Wang, W., Jahn, D. & Deckwer, W.-D. (2006)
Bacillus megaterium als Produktionssystem für rekombinante Proteine
Chemie Ingenieur Technik 78:289-294.