The influence of different nutrient and stress conditions on the quorum sensing (QS) and quorum quenching (QQ) regulated activities of Pseudomonas putida IsoF will be studied using physiologically defined chemostate conditions (different macronutrient starvation, challenging temperature and oxygen levels) as well as biofilm and rhizosphere colonization experiments. The QS-regulated putisolvin-production (psoR) and the expression of ppuI- and ppuR-gfp promotor fusions will be studied under these conditions together with the quantification of the N-acylhomoserine lactone (HSL) and homoserine (HS) molecules using high resolving metabolite analysis as well as monoclonal antibodies and ELISA-technique.
References:
- List of publications (A. Hartmann)
Contact details:
Prof. Dr. Anton Hartmann
Helmholtz Zentrum München, German Research Center for Environmental Health (GmbH)
Research Unit Microbe-Plant Intercations
Ingolstaedter Landstrasse 1
D-85764 Neuherberg
Tel.: +49-(0)89-31874109
Fax: +49-(0)89-31873382
anton.hartmann(at)helmholtz-muenchen.de
Homepage Link
Co-workers:
- Dr. Michael Rothballer
- Dr. Katharina Buddrus-Schiemann
- Dr. Martin Rieger
- Marlene Mühlbauer
References:
Hartmann
Hense, B. A., Müller, J., Kuttler, C. and Hartmann, A. (2012) Spatial heterogeneity of autoinducer regulation systems. Sensors (Basel), 12, 4156-4171.
Chen, X., Buddrus-Schiemann, K., Rothballer, M., Krämer, P. M. and Hartmann, A. (2010a) Detection of quorum sensing molecules in Burkholderia cepacia culture supernatants with enzyme-linked immunosorbent assays. Anal Bioanal Chem, 398, 2669-2676.
Chen, X., Kremmer, E., Gouzy, M.-F., Clausen, E., Starke, M., Wöllner, K., Pfister, G., Hartmann, A. and Krämer, P. M. (2010b) Development and characterization of rat monoclonal antibodies for N-acylated homoserine lactones. Anal Bioanal Chem, 398, 2655-2667.
Fekete, A., Kuttler, C., Rothballer, M., Hense, B. A., Fischer, D., Buddrus-Schiemann, K., Lucio, M., Müller, J., Schmitt-Kopplin, P. and Hartmann, A. (2010a) Dynamic regulation of N-acyl-homoserine lactone production and degradation in Pseudomonas putida IsoF. FEMS Microbiol. Ecol., 72, 22-34.
Fekete, A., Rothballer, M., Hartmann, A. and Schmitt-Kopplin, P. (2010b) Identification of bacterial autoinducers. In Bacterial SignalingWiley Publishers, Weinheim, Germany, pp. 95-111.
von Rad, U., Klein, I., Dobrev, P. I., Kottova, J., Zazimalova, E., Fekete, A., Hartmann, A., Schmitt-Kopplin, P. and Durner, J. (2008) Response of Arabidopsis thaliana to N-hexanoyl-DL-homoserine-lactone, a bacterial quorum sensing molecule produced in the rhizosphere. Planta, 229, 73-85.
Hense, B. A., Kuttler, C., Müller, J., Rothballer, M., Hartmann, A. and Kreft, J.-U. (2007) Does efficiency sensing unify diffusion and quorum sensing? Nat. Rev. Microbiol., 5, 230-239.
Fekete, A., Frommberger, M., Rothballer, M., Li, X., Englmann, M., Fekete, J., Hartmann, A., Eberl, L. and Schmitt-Kopplin, P. (2007) Identification of bacterial N-acylhomoserine lactones (AHLs) with a combination of ultra-performance liquid chromatography (UPLC), ultra-high-resolution mass spectrometry, and in-situ biosensors. Anal Bioanal Chem, 387, 455-467.
Götz, C., Fekete, A., Gebefuegi, I., Forczek, S. T., Fuksová, K., Li, X., Englmann, M., Gryndler, M., Hartmann, A., Matucha, M., Schmitt-Kopplin, P. and Schröder, P. (2007) Uptake, degradation and chiral discrimination of N-acyl-D/L-homoserine lactones by barley (Hordeum vulgare) and yam bean (Pachyrhizus erosus) plants. Anal Bioanal Chem, 389, 1447-1457.
Li, X., Fekete, A., Englmann, M., Götz, C., Rothballer, M., Frommberger, M., Buddrus, K., Fekete, J., Cai, C., Schröder, P., Hartmann, A., Chen, G. and Schmitt-Kopplin, P. (2006) Development and application of a method for the analysis of N-acylhomoserine lactones by solid-phase extraction and ultra high pressure liquid chromatography. J Chromatogr A, 1134, 186-193.
Frommberger, M., Hertkorn, N., Englmann, M., Jakoby, S., Hartmann, A., Kettrup, A. and Schmitt-Kopplin, P. (2005) Analysis of N-acylhomoserine lactones after alkaline hydrolysis and anion-exchange solid-phase extraction by capillary zone electrophoresis-mass spectrometry. Electrophoresis, 26, 1523-1532.