In a combined theoretical and experimental approach we plan to study heterogeneous gene expression of the Escherichia coli Colicin E2 operon in individual cells. Using fluorescence time-lapse microscopy we aim to quantitatively analyse the kinetics of Colicin expression from the Colicin operon and investigate further factors such as small RNAs regulating Colicin release and its impact on the establishment of heterogeneity. In particular, we are interested in changes of expression dynamics in dependence of SOS response and how this affects the amount and time-point of Colicin release.
References:
- List of publications (E. Frey)
- List of publications (M. Opitz)
Contact details:
Theoretical Modelling
Prof. Dr. Erwin Frey
Arnold-Sommerfeld-Cente for Theoretical Physics
Ludwig-Maximillians-Universität
Theresienstrasse 37
D-80333 München
Tel.: +49-(0)89-2180-4538
Fax: +49-(0)89-2180-4154
frey(at)lmu.de
Homepage Link
Experiment
Dr. Madeleine Opitz
Department für Physik
Chair Prof. Rädler
Ludwig-Maximillians-Universität
Geschwister-Scholl-Platz 1
D-80539 München
Tel.: +49-(0)89-2180-6760
Fax.:+49-(0)89-2180-3182
Opitz(at)physik.uni-muenchen.de
Homepage Link
Co-workers:
- Andreas Mader
- Matthias Schwarz
- Matthias Lechner
- Alexandra Götz
References:
Frey
Mader, A., Bronk, B. v., Ewald, B., Kesel, S., Schnetz, K., Frey, E. and Opitz, M. (2015) Amount of colicin release in Escherichia coli is regulated by lysis gene expression of the colicin E2 operon. PLoS ONE, 10, e0119124.
Melbinger, A., Cremer, J. and Frey, E. (2015) The emergence of cooperation from a single mutant during microbial life cycles. J R Soc Interface, 12.
Wienand, K., Lechner, M., Becker, F., Jung, H. and Frey, E. (2015) Non-Selective Evolution of Growing Populations. PLoS ONE, 10, e0134300.
Weber, M. F., Poxleitner, G., Hebisch, E., Frey, E. and Opitz, M. (2014) Chemical warfare and survival strategies in bacterial range expansions. J R Soc Interface, 11, 20140172.
Rulands, S., Jahn, D. and Frey, E. (2014) Specialization and bet hedging in heterogeneous populations. Phys. Rev. Lett., 113, 108102.
Hebisch, E., Knebel, J., Landsberg, J., Frey, E. and Leisner, M. (2013) High variation of fluorescence protein maturation times in closely related Escherichia coli strains. PLoS ONE, 8, e75991.
Cremer, J., Melbinger, A. and Frey, E. (2012) Growth dynamics and the evolution of cooperation in microbial populations. Sci Rep, 2, 281.
Melbinger, A., Cremer, J. and Frey, E. (2010) Evolutionary game theory in growing populations. Phys. Rev. Lett., 105, 178101.
Leisner, M., Kuhr, J.-T., Rädler, J. O., Frey, E. and Maier, B. (2009) Kinetics of genetic switching into the state of bacterial competence. Biophys. J., 96, 1178-1188.
Leisner, M., Stingl, K., Frey, E. and Maier, B. (2008) Stochastic switching to competence. Curr. Opin. Microbiol., 11, 553-559.
Opitz
Mader, A., Bronk, B. v., Ewald, B., Kesel, S., Schnetz, K., Frey, E. and Opitz, M. (2015) Amount of colicin release in Escherichia coli is regulated by lysis gene expression of the colicin E2 operon. PLoS ONE, 10, e0119124.
Weber, M. F., Poxleitner, G., Hebisch, E., Frey, E. and Opitz, M. (2014) Chemical warfare and survival strategies in bacterial range expansions. J R Soc Interface, 11, 20140172.
Hebisch, E., Knebel, J., Landsberg, J., Frey, E. and Leisner, M. (2013) High variation of fluorescence protein maturation times in closely related Escherichia coli strains. PLoS ONE, 8, e75991.
Kesel, S., Mader, A., Höfler, C., Mascher, T. and Leisner, M. (2013) Immediate and Heterogeneous Response of the LiaFSR Two-Component System of Bacillus subtilis to the Peptide Antibiotic Bacitracin. PLoS ONE, 8, e53457.
Herbst, K., Bujara, M., Heroven, A. K., Opitz, W., Weichert, M., Zimmermann, A. and Dersch, P. (2009) Intrinsic thermal sensing controls proteolysis of Yersinia virulence regulator RovA. PLoS Pathog., 5, e1000435.
Leisner, M., Kuhr, J.-T., Rädler, J. O., Frey, E. and Maier, B. (2009) Kinetics of genetic switching into the state of bacterial competence. Biophys. J., 96, 1178-1188.
Leisner, M., Stingl, K., Frey, E. and Maier, B. (2008) Stochastic switching to competence. Curr. Opin. Microbiol., 11, 553-559.
Leisner, M., Stingl, K., Rädler, J. O. and Maier, B. (2007) Basal expression rate of comK sets a 'switching-window' into the K-state of Bacillus subtilis. Mol. Microbiol., 63, 1806-1816.