TU BRAUNSCHWEIG

 

Welcome to the Research Group of Philip Tinnefeld

Institute of Physical and Theoretical Chemistry, TU Braunschweig
Braunschweig Integrated Center of Systems Biology (BRICS)
Laboratory for Emerging Nanometrology (LENA)

Rebenring 56

38106 Braunschweig

Email: p.tinnefeld@tu-braunschweig.de
Phone: +49 531 391 55243
Fax: +49 531 391 55250


We moved into a new building: BRICS, Rebenring 56, 38106 Braunschweig


Award: GATTAquannt GmbH wins first prize in the OptecNet Start-up Challenge (22.06.2016). Congrats to Jürgen, Carsten, Max and Soefiene.


Teaching: PCI-Klausur im Sommersemester 2016: Infos hier


New Publication: C. Vietz, B. Lalkens, G.P. Acuna, P. Tinnefeld (2016): Functionalizing large nanoparticles for small gaps in dimer nanoantennas - New Journal of Physics, online.


New Publication: H.C. Høiberg, S.S. Simmel, P. Holzmeister, P. Tinnefeld, T. Liedl, P.C. Nickels (2016): DNA Origami Seesaws as Comparative Binding Assay - ChemBioChem, 17, 1-5.


New Publication: S. Schulz, A. Gietl, K. Smollett, P. Tinnefeld, F. Werner, D. Grohmann (2016): TFE and Spt4/5 open and close the RNA polymerase clamp during the transcription cyclePNAS, 113, 13, E1816-1825, press release

New Publication: E. A. Hemmig, C. Creatore, B. Wünsch, L. Hecker, P. Mair, M. A. Parker, S. Emmott, P. Tinnefeld, U. F. Keyser, and A. W. Chin (2016): Programming light-harvesting efficiency using DNA origami - Nano Lett., 16, 4, 2369-2374.


Teaching: LabVIEW-workshop starts on 5th April 2016, 11:30-13:00. Further information on registration and deadline here.


Teaching: Die Modulabschlussklausur aus PC1 und PC2 (für Chemiker) 19.02.2016: hier; Ergebnisse


We welcome Dr. Izabela Kaminska  from Nicolaus Copernicus University/Poland as PostDoc in our research group!


New Publication: J. Molle, M. Raab, S. Holzmeister, D. Schmitt-Monreal, D. Grohmann, Z. He, P. Tinnefeld (2016): Superresolution microscopy with transient binding - Curr Opin Biotech, 39, 8-16.


Publication: J. Chen, X. Ji, P. Tinnefeld, Zhike He (2016): Multifunctional Dumbbell-Shaped DNA-Templated Selective Formation of Fluorescent Silver Nanoclusters or Copper Nanoparticles for Sensitive Detection of Biomolecules, ACS Appl. Mater. & Interf.  8, 3, 1786-1794.


Publication: A. Puchkova, C.Vietz, E. Pibiri, B. Wuensch, M. Sanz Paz, G. P. Acuna, P. Tinnefeld (2015): DNA Origami Nanoantennas with over 5000fold Fluorescence Enhancement and Single-Molecule Detection at 25 µM - Nano Lett., 15(12), 8354-8359.


Award: Our team „Nanolabor“ wins second prize in the Bio-Gründer Competition 2015. BIO-Security. Congrats to Birka with Guillermo, Sofiene, Caro and Philip. Press release/BRICS-news


We welcome Prof. Baoxiang Gao from Hebei University/China as Visiting Professor in our research group!


Teaching: Die Klausureinsicht für PC1 für alle Studiengänge findet am Dienstag 01.09.2015 um 10 Uhr, Hans-Sommer-Straße 10, 2. OG statt.

Ergebnisse: hier.


Publication: H. Ta, J. Keller, M. Haltmeier, S.K. Saka, J. Schmied, F. Opazo, P. Tinnefeld, A. Munk, S. Hell (2015): Mapping molecules in scanning far-field fluorescence nanoscopy - Nat. Commun., 6:7977.


Teaching: Lecture "Moderne optische Methoden und Imaging" starts 2015, October 21st, 14:30 / HS10, room 219. All those  interested in the lecture should  register by  contacting Prof. Tinnefeld: p.tinnefeld@tu-braunschweig.de

For more information: here


Publication: S. W. Hell, S. J. Sahl, M. Bates, X. Zhuang, R. Heintzmann, M. J Booth, J. Bewersdorf, G. Shtengel, H. Hess, P. Tinnefeld, A. Honigmann, S. Jakobs, I. Testa, Cognet, B. Lounis, H. Ewers, S. J Davis, C. Eggeling, D. Klenerman, K. I Willig, G. Vicidomini, M. Castello, A. Diaspro and T. Cordes (2015): The 2015 super-resolution microscopy roadmap - J. Phys. D: Appl. Phys., 48:443001.


Teaching: Die Klausur Physikalische Chemie I findet am 21.08.2015 statt. Für weitere Informationen hier


We welcome Prof. Zhike He from Wuhan University/China as Visiting Professor in our research group!


Publication: T. Cordes, J. Vogelsang, C. Steinhauer, I.H. Stein, C. Forthmann, A. Gietl, J. Schmied, G. Acuna, S. Laurien, B. Lalkens, P. Tinnefeld (2015): Far-Field Nanoscopy with Conventional Fluorophores: Photostability, Photophysics & Transient Binding - Springer Series on Fluorescence, Volume on “Far-Field Optical Nanoscopy”, Series Editor: O. Wolfbeis, Volume Editors: P. Tinnefeld, C. Eggeling, S.W. Hell, online.


Publication: I. Jusuk, C. Vietz, M. Raab, T. Dammeyer, P. Tinnefeld (2015): Super-Resolution Imaging Conditions for enhanced Yellow Fluorescent Protein (eYFP) Demonstrated on DNA Origami Nanorulers - Sci. Rep., 5:14075.


Publication: T. Ye, C. Li, C. Su, X. Ji, J. Zheng, P. Tinnefeld, Zhike He (2015): Enzymatic Polymerization of Poly (Thymine) for Copper Nanoparticles Synthesis with Tunable Size and Its Application in Enzyme Sensing - Chem. Comm., 51, 8644-8647.


Publication: P. Ringel, C. Probst, T. Dammeyer, S. Buchmeier, L. Jänsch, J. Wissing, P. Tinnefeld, R.R. Mendel, B.M. Jockusch, T. Kruse (2015): Enzymatic characterization of recombinant nitrate reductase expressed and purified from Neurospora crassa - Fungal Genetics and Biology, 80, 10-18.


Publication: C. Kaplan, B. Jing, C. Winterflood, D. Bridges, P. Occhipinti, J.J. Schmied, S. Grinhagens, T. Gronemeyer, P. Tinnefeld, A. Gladfelter, J. Ries, H. Ewers (2015): Absolute Arrangement of Subunits in Cytoskeletal Septin Filaments in Cells Measured by Fluorescence Microscopy - Nano Lett., 15(6), 3859-3864.


Congratulations to Dr. Dina Grohmann for her new position as Professor for Biology at Regensburg university!


Teaching: LabVIEW-workshop starts on 14 April 2015. Further information on registration and deadline here.


Publication: S. Beater,  P. Holzmeister, E. Pibiri, B. Lalkens, and P. Tinnefeld (2015): Simple and aberration-free 4color-STED - multiplexing by transient binding - Optics Express, 23(7), 8630-8638.


Teaching: PC I-Thermodynamics lecture starts after Easter, on 17 April 2015, 8 a.m. PK 2.1.


Award: E.G. Rochow Family Trust prize awarded to Dr. Phil Holzmeister!


Klausurergebnisse: PC1 und PC Modul jetzt hier


Poster award: Dr. Dina Grohmann was awarded first place for her poster presentation at the 50th Winter Seminar on "Biophysical Chemistry, Molecular Biology and Cybernetics of Cell Functions"


Klausurankündigung: Die PC-Modulklausur und die PC1-Klausur finden am 13.2.2015 um 13.30 in den Räumen Zi24.1 und Zi24.2 statt. Siehe auch hier.


BIOMOD: The Nanoscooter won the gold medal in the big jamboree hostet by the WYSS institute in Harvard!


Publication: P. Holzmeister, E. Pibiri, J.J. Schmied, T.Sen, G.P. Acuna, P. Tinnefeld (2014): Quantum yield and excitation rate of single molecules close to metallic nanostructures - Nature Communications, 5:5356 press release 


BRICS news: BRICS is a joint center of the TU Braunschweig and the Helmholtz Center for Infection Research. All up-to-date information here.


Teaching: Lecture "Moderne optische Methoden und Imaging" starts 2014, October 22th, 13:30 / HS10.1


New Book: "Far-Field Optical Nanoscopy" Springer Series on Fluorescence Volume 14 edited by Christian Eggeling, Stefan Hell, and Philip Tinnefeld. Available as ebook (see chapters) and hardcover on 24.03.2015.


Publication: A. Gust, A. Zander, A. Gietl, P. Holzmeister, S. Schulz, B. Lalkens, P. Tinnefeld, D. Grohmann (2014): A Starting Point for Fluorescence-Based Single-Molecule Measurements in Biomolecular Research, Molecules, 19, 10, 15824-15865.


Press release: Photonik Forschung Deutschland (supported by BMBF) highlights the foundation of the startup company GATTAquant. For more information here.


Ergebnisse der PC1-Klausur jetzt online!


Publication: G.P. Acuna, D. Grohmann, P. Tinnefeld (2014): Enhancing Single-Molecule Spectroscopy with Nanophotonics, Minireview - FEBS Lett., 588, 19. 3547-3552


Publication: S. Beater, M. Raab, P. Tinnefeld (2014):  Toward quantitative fluorescence microscopy with DNA origami nanorulers (Book Chapter)Methods in Cell Biology Book Series, online.


Publication: M. Raab, J.J. Schmied, I. Jusuk, C. Forthmann, P. Tinnefeld (2014): Fluorescence Microscopy with 6 nm Resolution on DNA Origami - ChemPhysChem, 15(12), 2431-2435, (issue back cover).


Publication: J.J. Schmied, M. Raab, C. Forthmann, E. Pibiri, B. Wünsch, T. Dammeyer, P. Tinnefeld (2014): DNA origami based standards for quantitative fluorescence microscopy - Nature Prot., 9, 1367-1391


Publication: E. Pibiri, P. Holzmeister, B. Lalkens, G.P. Acuna, P. Tinnefled (2014): Single-Molecule Positioning in Zeromode Waveguides by DNA Origami Nano-Adapters - Nano Lett., 14(6), 3499–3503. Press release.


Publication: P. Holzmeister, A. Gietl, and P. Tinnefeld (2014)Geminate Recombination as a Photoprotection Mechanism for Fluorescent Dyes - Angew. Chem. Int. Ed., 126, 22, 5792-5796, hot paper.  Press release / BRICS news.


Publication: A. Gietl, P. Holzmeister, F. Blombach, S. Schulz, L. Voith von Voithenberg, D. C. Lamb, F. Werner, P. Tinnefeld and D. Grohmann (2014): Eukaryotic and archaeal TBP and TFB/TF(II)B follow different promoter DNA bending pathways - Nucleic Acids Research, 42(10):6219-31.Access the recommendation on F1000Prime


Publication: J.V. Pellegrotti, G.P. Acuna, A. Puchkova, P. Holzmeister, A. Gietl, B. Lalkens, F. Stefani, P. Tinnefeld (2014): Controlled reduction of photobleaching in DNA origami – gold nanoparticle hybrids - Nano Lett., 14(5), 2831-2836. Press release / BRICS news.


Vacancies: We are seeking a business economist. For further information on employment ad click here.


Teaching summer term 2014:

  • LabVIEW-course starts on 7 April 2014. Further information on registration and deadline here.
  • PC I-Thermodynamics lecture starts after Easter, on 23 April 2014!

Klausurergebnisse: PC1/PC-Modul.

Die  Klausureinsicht findet am Mittwoch, den 5.3.2014 von 9.30 bis 11.30 im Raum 219 statt.


Publication: S. Beater,  P. Holzmeister, E. Pibiri, B. Lalkens, and P. Tinnefeld (2014): Choosing Dyes for cw-STED Nanoscopy using self-assembled Nanorulers - PhysChemChemPhys, 16, 6990-6996.


Exploration Grant: Dina Grohman receives  an "Exploration Grants for outstanding research group leaders" from the Boehringer Ingelheim Stiftung (2014).


Publication: A. Zander, P. Holzmeister, Klose D., P. Tinnefeld and D. Grohmann (2014): Single-molecule FRET supports two-state model of Argonaute actionRNA Biology, 11(1), 45-56.


Publication: P.Holzmeister, B. Wünsch, A. Gietl, P. Tinnefeld (2014): Single-Molecule Photophysics of Dark Quenchers as Non-Fluorescent FRET acceptors - Photochem. & Photobiol. Sci., 2014,13, 853-858.


Publication: S. Heucke, F. Baumann, G.P. Acuna, P.M.D. Severin, S.W. Stahl, M. Strackharn, I.H. Stein, P. Altpeter, P. Tinnefeld, H.E. Gaub (2014): Placing Individual Molecules in the Center of Nanoapertures - Nano Lett., 14(2), 391-395.


Two Publications won a CeNS Publication Award 2013:

  • Placing Individual Molecules in the Center of Nanoapertures - Nano Letters (online) is a winner of the CeNS Publication Award 2013!
  • DNA Origami Nanopillars as Standards for Three-dimensional Superresolution Microscopy - Nano Letters13(2), 781–785 is a winner of the CeNS Publication Award 2013!

Publication: P. Holzmeister, G. P. Acuna, D. Grohmann, P. Tinnefeld (2014): Breaking the Concentration Limit of Optical Single -Molecule Detection - Chem.Soc.Rev., 43(4), 1014-1028 (issue back cover).

This is a HOT Chem.Soc.Rev. article! Download for free untill 20th October 2013!


Teaching: Lecture "Advanced Physical Chemistry: NanoBioSciences" takes place on Wednesdays and Thursdays, 8:15 - 9:45 s.t. / HS10.1.


Publication: X. Yu, D.Y. Lei, F. Amin, R. Hartmann, G.P. Acuna, A. Guerreo-Martinez, S.A. Maier, P. Tinnefeld, S. Carregal-Romero, W.J. Parak (2013): Distance Control in-between Plasmonic Nanoparticles via Biological and Polymeric SpacersNano Today, 8 (5), 480-493.


Teaching: "Advanced Physical Chemistry: NanoBioSciences" starts 2013, October 24th, 9:00 s.t. / HS10.1


Poster award: Phil Holzmeister was awarded the poster prize for the single molecule session at the International Conference on Photochemistry 2013


Klausur Biophysikalische Chemie: aktuelle Informationen ab sofort hier.


CeNS Award: Friedericke Möller wins Attocube Research Award 2013  for Master thesis!


Klausur PC1 und Modulklausur PC1+PC2: aktuelle Informationen ab sofort hierAchtung: Änderung des Raums!


Publication: F.M. Möller, P. Holzmeister, T. Sen, G.P. Acuna, P. Tinnefeld (2013): Modulation of Single-Molecule Fluorescence by Gold Nanoparticles on DNA Origami Templates - Nanophotonics, 9 (13), 2210-2222.


Publication: A. Kurz, J.J. Schmied, K. Grußmayer, P. Holzmeister, P. Tinnefeld, D.-P. Herten (2013): Counting Fluorescent Dye Molecules on DNA Origami by Means of Photon Statistics - Small, 9 (23), 4061-8.


Publication: D. Grohmann, F. Werner, P. Tinnefeld (2013): Making Connections–Strategies for Single-Molecule Fluorescence Biophysics - Curr. Op. Chem. Biol., 17, online.


Publication: P. Tinnefeld (2013): Single-Molecule Detection: Breaking the Concentration Barrier - Nature Nanotechnol., 8, 480–482.


Publication: G.P. Acuna, P. Holzmeister, F.M. Möller, S. Beater, B. Lalkens, P. Tinnefeld (2013): DNA-Templated Nanoantennas for Single-Molecule Detection at Elevated Concentrations - J. Biomed. Opt., 18, 6, 065001, 1-5.


Publication: T. Dammeyer, K.N. Timmis, P. Tinnefeld (2013): Broad Host Range Vectors for Expression of Proteins with (Twin-) Strep-Tag, HIS-Tag and Engineered, Export Optimized Yellow Fluorescent Protein - Microbial Cell Factories, 12, 1, 49.


Publication: S. Heucke, E. Puchner , S. Stahl, A. Holleitner, H. E. Gaub, P. Tinnefeld (2013): Nanoapertures for AFM-based Single-Molecule Force Spectroscopy - Int. J. Nanotechnol., 10, 5/6/7, 607-619.


Teaching: The block course "Angewandte Biophysikalische Chemie" for Chemistry and Biotechnology students takes place at 5.4.2013. Details.


Highlights: Acuna et al. (Science 2012) has been highligted in several jounals:


Publication: A. Gietl and D. Grohmann (2013):  Modern biophysical approaches probe transcription-factor-induced DNA bending and looping - Biochem Soc Trans., 1;41(1):368-73.


Publication: J.J. Schmied, C. Forthmann, E. Pibiri, B. Lalkens, P. Nickels, T. Liedl and P. Tinnefeld (2013): DNA Origami Nanopillars as Standards for Three-dimensional Superresolution Microscopy - Nano Letters13(2), 781–785.


Publication:  C. Steinhauer, M.S. Itano and P. Tinnefeld (2013): Super-Resolution Fluorescence Imaging with Blink Microscopy - Methods in Molecular Biology, 950, 111-129, Springer Protocolls, Book Title: Nanoimaging: Methods and Protocols.


Publication: J.J. Schmied, A. Gietl, P. Holzmeister, C. Forthmann, C. Steinhauer, T. Dammeyer and P. Tinnefeld (2012): Fluorescence and super-resolution standards based on DNA origami - Nature Methods, 9, 1133–1134, Press release: German / English 


Publication: T. Dammeyer and P. Tinnefeld (2012): Engineered fluorescent proteins illuminate the bacterial periplasm - Computational and Structural Biotechnology Journal.


CeNS Award: Dr. Guillermo Acuna won a CeNS Publication Award 2012


Publication: G.P. Acuna, F.M. Möller, P. Holzmeister, S. Beater, B. Lalkens and P. Tinnefeld (2012): Fluorescence Enhancement at Docking Sites of DNA-Directed Self-Assembled Nanoantennas - Science, 338(6106): 506-510,  Press release, Research Highlight, News & Views


Teaching  in winter term 2012/13: 


Publication: T. Cordes, J. Vogelsang, C. Steinhauer, I.H. Stein, C. Forthmann, A. Gietl, J. Schmied, G. Acuna, S. Laurien, B. Lalkens, P. Tinnefeld (2012): Far-Field Nanoscopy with Conventional Fluorophores: Photostability, Photophysics & Transient Binding - Springer Series on Fluorescence, Volume on “Far-Field Optical Nanoscopy”, Series Editor: O. Wolfbeis, Volume Editors: P. Tinnefeld, C. Eggeling, S.W. Hell.


GRC poster award: Phil Holzmeister was awarded second place for his poster presentation at the Gordon Research Conference on "Single Molecule Approaches to Biology".


Publication: P. Tinnefeld, T. Cordes (2012): ‘Self-Healing Dyes’ – Intramolecular Stabilization of Organic Fluorophores - Nat. Methods, 9: 426–427.


Publication: A. Gietl, P. Holzmeister, D. Grohmann, P. Tinnefeld (2012): DNA Origami as Biocompatible Surface to Match Ensemble and Single-Molecule Experiments - Nucleic Acids Res., 40, 14, e110.


Teaching: PC I: Thermodynamics lecture starts at 11. April 2012. An overview of the lecture will be provided and organizational issues regarding the lecture as well as the tutorials will be discussed.


Publication: M.S. Itano, C. Steinhauer, J. Schmied, C. Forthmann, P. Liu, A.K. Neumann, K. Jacobson, P. Tinnefeld, N. Thompson (2012): Super-Resolution Imaging of C-Type Lectin and Influenza Hemagglutinin Nanodomains on Plasma Membranes using Blink Microscopy - Biophys. J., 102(7): 1534-1542.


Publication: G.P. Acuna, M. Bucher, I.H. Stein, C. Steinhauer, A. Kuzyk, P. Holzmeister, R. Schreiber, A. Moroz, F.D. Stefani, T. Liedl, F.C. Simmel, P. Tinnefeld (2012): Distance Dependence of Single-Fluorophore Quenching by Gold Nanoparticles studied on DNA Origami - ACS Nano,  6, 4, 3189-3195.


Publication: T. Ha and P. Tinnefeld (2012): Photophysics of Fluorescence Probes for Single-Molecule Biophysics and Super-Resolution Imaging - Annu. Rev. Phys. Chem., 63, 26.1 - 26.23

Publcation: Ingo H. Stein, Stella Capone, Jochem H. Smit, Fabian Baumann, Thorben Cordes and Philip Tinnefeld (2012): Linking Single-Molecule Blinking to Chromophore Structure and Redox Potentials - ChemPhysChem., 13(4):931-937.


CeNS Award: Ingo Stein and Christian Steinhauer won a CeNS Publication Award  2011!

  • Ingo H. Stein, Verena Schüller, Philip Böhm, Philip Tinnefeld, and Tim Liedl (2011): Single-Molecule FRET Ruler Based on Rigid DNA Origami Blocks ChemPhysChem, 12(3): 689-695.
  • Ralf Jungmann, Christian Steinhauer, Max Scheible, Anton Kuzyk, Philip Tinnefeld, and Friedrich Simmel (2010): Single-Molecule Kinetics and Super-Resolution Microscopy by Fluorescence Imaging of Transient Binding on DNA Origami - Nano Lett. 10(11): 4756–4761

Teaching WiSe 2011/12: The "Seminar zum Praktikum Physikalische Chemie (BSc)" starts on 25.10.2011 (details here)


Teaching WiSe 2011/12: Lecture "Advanced Physical Chemistry - NanoBioSciences" starts on 27.10.2011 (details here!)


Publication: Carsten Forthmann, Jürgen Schmied and Philip Tinnefeld (2011): Super-auflösende Fluoreszenzmikroskopie mit einzelnen Molekülen - LaborPraxis


ChemCup 2011: Winner of the soccer tournament is the "Bunter Haufen" team (Tinnefeld group and friends)!


Teaching: Please register here for the OC/PC-Practicum WS 2011/12.


Publication: D. Smith, V. Schüller, C. Forthmann, R. Schreiber, P. Tinnefeld, T. Liedl (2011): A structurally variable hinged tetrahedron framework from DNA origami - Journal of Nucleic Acid, ID 360954


Publication: I.H. Stein, P. Tinnefeld (2011): Single-Molecule FRET & Super-Resolution: DNA Nanostructures Organize Dyes for Nanophotonic Apps - GIT Imaging and Microscopy


Join us: Vacancies (BSc, MSc, PhD, PostDoc)


Teaching: PC I: Thermodynamics lecture starts at 6. April 2011. An overview of the lecture will be provided and organizational issues regarding the lecture as well as the tutorials will be discussed.


Publication: I.H. Stein, C. Steinhauer and P. Tinnefeld (2011): Single-Molecule Four-Color FRET Visualizes Energy Transfer Paths on DNA Origami - J Am Chem Soc. Issue cover.
Highlighted in Nature Chemistry: "Energy transfer: On the right path" by Bo Albinsson. Also highlighted in academics.de and pro-physik.de.


Teaching: WiSe2010/11 The block course "Angewandte Biophysikalische Chemie" takes place 11.3.2011 from 9am to 2pm in HR 30.2.


Publication: T. Cordes, A. Maiser, C. Steinhauer, L. Schermelleh, P. Tinnefeld (2011): Mechanism and Advancement of Antifading Agents for Confocal Microscopy - PhysChemChemPhys


Publication: Ingo H. Stein, Verena Schüller, P. Böhm, P. Tinnefeld, T. Liedl (2011): Single-molecule FRET ruler based on rigid DNA origami blocks - ChemPhysChem


Upcoming event: Braunschweiger Bio-Imaging Symposium BBIS, 16.02.2011, 1:20 pm – 6:00 pm, Neuer Senatssitzungssaal. Download symposium program here!


Christian Steinhauer won the „Student presentation award  of the „Australian Intsitut of Physics Congress 2010“.


Nano meets Bio: Prof. Tinnefeld's inaugural lecture


Publication: R. Jungmann, C. Steinhauer, M. Scheible, A. Kuzyk, P. Tinnefeld, F.C. Simmel (2010): Single-Molecule Kinetics and Super-Resolution Microscopy by Fluorescence Imaging of Transient Binding on DNA Origami - Nano Letters


Teaching in winter term 2010: Biophysical Chemistry (M.Sc.Chem.)


Old group homepage at the LMU: http://www.biophysik.physik.uni-muenchen.de/groups/single-molecule-fluorescence-spectroscopy


Having started Aug. 1, 2010...

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