Experimental analysis and mathematical modelling of mechano-regulated growth and remodelling processes in urinary bladders of post-pubescent pigs
The urinary bladder is a central organ of vertebrates. Its primary function, the storage of significant volumes of urine, implies very large deformations, which leads to an important role of mechanics in the urinary bladder. In particular growth and remodeling processes controlled by mechanical stimuli play an important role both in health and disease. For example, partial outlet obstruction of the bladder – from which 50% to 75% of the male population older than 50 years suffer – frequently results in pathological growth and remodeling with consequences such as ischuria. However, the biomechanical foundations of mechano-regulated growth and remodelling in the bladder remain poorly understood to date. This project aims to understand and quantify mechano-regulated growth and remodelling processes in the urinary bladder. To this end, a series of biomechanical and immunohistochemical experiments with urinary bladders of post-pubescent pigs of different ages will be conducted. The results of these experiments will serve as a basis for the development of the world-wide first mathematical/computational model of mechano-regulated growth and remodelling in the urinary bladder. To exclude confounding effects of hormones, their concentrations will be measured and incorporated appropriately in the model. The mathematical model will be based on the theory of constrained mixtures. Its parameters and functional relations will be determined from the experimental data using a sequential Monte-Carlo method. Finally, the model will be used to study for the first time with a very simple computational model mechano-regulated growth and remodelling in case of a partial outlet obstruction of the urinary bladder. This way, the applicability of the results of this project to clinically relevant questions will be demonstrated.
Although this project mainly focuses on the relation between mechanical stimuli and growth and remodeling, it will, as a byproduct, also reveal for the first time how hormonal and mechanical factors superimpose in the controlling growth and remodeling in the urinary bladder.
2019
A. E. Ehret, M. Böl
Recent topics in biomechanics and mechanobiology
GAMM Mitteilungen, 1, (2019) [Link]
M. Borsdorf, A. Tomalka, N. Stutzig, E. Morales Orcajo, M. Böl, T. Siebert
Locational and directional dependencies of smooth muscle properties in pig urinary bladder
Frontiers in Physiology, 10, 63, (2019) [Link]
2018
R. Seydewitz, E. Morales-Orcajo, T. Siebert, M Böl
On a three-dimensional mechano-electrochemical model for smooth muscle
8th World Congress of Biomechanics, Dublin (Ireland), July 2018
Development and validation of a constitutive growth model for brain tissue characterising brain alterations in space and time
The brain, as a part of the central nervous system is probably the most complex biological system, which undergoes significant changes, especially during its growth phase. Because of these complexities, both, at the macroscopic and microscopic level, and the associated difficulties in experimental sampling, there are insufficient experimental investigations. In addition, age-related, mechanical investigations on brain tissue, which were also carried out spatially resolved, are extremely rare.However, such experiments are essential in order to better understand the mechanical properties and functions of the brain during growth and to be able to develop and validate numerical models.The performance of such models in terms of predictive accuracy depends particularly on the quality of the identified material parameters.
The objective of this research project is the development and validation of a constitutive growth model for brain tissue that characterises alterations of the mammalian brain both, in space and time. This goal will be achieved in four steps through a close interplay of experiment, modelling, and simulation. Experiments will be performed on porcine brain tissue, displaying similar microstructural and mechanical properties as the human brain. An essential requirement for the modelling is the method development to perform appropriate tissue-level experiments. Since grey and white matter tissue will be sampled individually, the tissue specimens are relatively small. This implies that for the axial, biaxial, and triaxial experiments planned within this project, micromechanical measurement setups under a microscope have to be developed, constructed, and verified. These measurements will allow us to accurately characterise the axial, biaxial, and triaxial behaviour of brain tissue. To examine the influence of neurodevelopment on the mechanical properties, brain samples will be collected at different ages. In a third step, the microstructure as well as the macroscopic geometry of the brain will be determined by means of immunohistological investigations. The resulting cell density, myelin content, and tissue microstructure will directly inform the model development and validation.
2019
A. E. Ehret, M. Böl
Recent topics in biomechanics and mechanobiology
GAMM Mitteilungen, 1, (2019) [Link]
Development of a three-dimensional model of structural and functional changes during skeletal muscle growth
During growth muscle tissue is subjected to extensive structural and mechanical changes. Thereby, the increase of muscle force is related to the adaptation of the three-dimensional muscle geometry and the material behaviour of the muscle. The whole growth process happens while retaining the muscle function. The essential condition for the understanding and the modelling of growth is the acquisition of the structural and mechanical changes. Aim of the project is the development and validation of a growth model for skeletal muscles. This aim is ensured by two interacting steps, that are characterised by a tight complexity between experiment and modelling.
Aim of the project is the development of a growth model for the simple structured rabbit m. soleus. Rooted on age-dependent experiments, specific muscle parameters will be identified. This experimental work comprises the determination of the active muscle characteristics, the passive tissue behaviour, the muscle deformation during contraction, the three-dimensional muscle architecture, as well as the immunohistologic analysis of the muscle tissue. Based on these experimental results, growth kinetics for various state variables will be identified, which will be used in a subsequent step for the development of the growth models. The model validation occurs on experimental force and deformation data that have been imposed from dynamic muscle contraction experiments for all muscle ages. In a second project phase the growth model will be verified on the much more complex m. plantaris. This multi-pennate muscle is characterised by a complex fibre architecture combined with inner tendon sheaths as well as an inhomogeneous fibre type distribution.
A successful development and validation of the growth models as planed in this project will accelerate the knowledge concerning the growth behaviour of skeletal muscles in a distinct way. The knowledge of structural changes and contractile characteristics is a crucial requirement for a deeper understanding of muscle shape changes and the differentiation during growth. An adequate growth model allows the studying of state variables inside the muscle as well as their change during growth. This will lead to a better understanding of biomechanical relations. Furthermore, the successful development of growth models is a basis to answer open evolutionary-biological questions like the three-dimensional arrangement (assembly of muscles in muscle packages) and the growth of extremities.
2020
P. Schenk, S. Papenkort, M. Böl, T. Siebert, R. Grassme, C. Rode
A simple geometrical model accounting for 3D muscle architectural changes across muscle lengths
Journal of Biomechanics, 103, 109694, (2020) [Link]
R. Rockenfeller, M. Günther, N. Stutzig, D. Haeufle, T. Siebert, S. Schmitt, K. Leichsenring, M. Böl, T. Götz
Exhaustion of skeletal muscle fibers within seconds: incorporating phosphate kinetics into a Hill-type model
Frontiers in Physiology, Striated Muscle Physiology, 11, 306, (2020) [Link]
S. Papenkort, M. Böl, T. Siebert
Three-dimensional muscle architecture of rabbit M. soleus during growth
submitted, (2020)
2019
A. E. Ehret, M. Böl
Recent topics in biomechanics and mechanobiology
GAMM Mitteilungen, 1, (2019) [Link]
M. Böl, R. Iyer, J. Dittmann, M. Garcés-Schröder, A. Dietzel
Investigating the passive mechanical behaviour of skeletal muscle fibres: Micromechanical experiments and Bayesian hierarchical modelling
Acta Biomaterialia, 92, 277-289, (2019) [Link]
R. Seydewitz, T. Siebert, M. Böl
On a three-dimensional constitutive model for history effects in skeletal muscles
Biomechanics and Modeling in Mechanobiology, 18, 1665-1681, (2019) [Link]
2018
C. Wick, M. Böl, Florian Müller, R. Blickhan, T. Siebert
Packing of muscles in the rabbit shank influences three-dimensional architecture of M. soleus
Journal of the Mechanical Behavior of Biomedical Materials, 83, 20-27, (2018) [Link]
2017
T. Siebert, A. Tomalka, N. Stutzig, K. Leichsenring, M. Böl
Changes in three-dimensional muscle structure of rabbit gastrocnemius, flexor digitorum longus, and tibialis anterior during growth
Journal of the Mechanical Behavior of Biomedical Materials, 74, 507-519, (2017) [Link]
M. Böl, K. Leichsenring, T. Siebert
Effects of growth on muscle, tendon and aponeurosis tissues in rabbit shank musculature
The Anatomical Record, 300, 1123-1136, (2017) [Link]
2019
M. Böl
Skeletal muscle mechanics – experiment and simulation
Paris-Saclay University Biomechanics Seminar Series, École Polytechnique, Palaiseau (France), October 2019
M. Böl
Aspects of active muscle modelling - multi-scale/field modelling in biomechanics
International Autumn School 2019, Berlin (Germany), October 2019
M. Böl
Three-dimensional multiscale/field modelling in muscle mechanics - Challenges in modelling and experiment
24th Sports Science University Day of the DVS, Berlin (Germany), September 2019
M. Böl
Multiscale/field modelling in muscle mechanics - experiment versus simulation
Technical University of Munich, Munich (Germany), February 2019
2018
R. Seydewitz, T. Siebert, M.Böl
On the phenomenological modelling of history effects in skeletal muscles
13th World Congress in Computational Mechanics, New York City (USA), July 2018
M. Böl
Biomechanics - Multi-scale/field experimental approaches
University of Kassel, Kassel (Germany), April 2018
2017
M. Böl
Advanced experiments on biological systems at different length scales
ETH Zurich, Zurich (Zwitzerland), December 2017
T. Siebert, A. Tomalka, K. Leichsenring, M. Böl
Changes in three dimensional muscle structure during growth
EUROMECH Colloquium 585, Warberg (Germany), February 2017
K. Leichsenring, T. Siebert, M. Böl
Skeletal muscle growth in rabbit shank musculature - an experimental study
EUROMECH Colloquium 585, Warberg (Germany), February 2017
2016
K. Leichsenring, T. Siebert, M. Böl
Experimental quantification of skeletal muscle growth in oryctolagus cuniculus
The 12th World Congress of Computational Mechanics (WCCM XII), Seoul (South Korea), July 2016
K. Leichsenring, T. Siebert, M. Böl
Muscle growth – an experimental study
European Congress on Computational Methods in Applied Sciences and Engineering (ECCOMAS), Crete Island (Greece), June 2016
Mechanical characterisation at cell level
Oocytes are cells, whose sizes are big enough to allow a micro-mechanical characterisation. In our research, we study porcine oocytes with a diameter of about 140 µm. The Zona pellucida is the membrane surrounding the ooplasm, whose thickness is about 14 µm. This membrane ensures that only one sperm can penetrate the cell, to prevent polyspermy. The glycoproteins in the membrane form filaments in approximately three layers. In literature different orientations of these filaments are discussed. For a better understanding of the mechanical behaviour, the anaylsis of the filament orientation is of major interest.
One possibility to determine the orientation of filaments is a technique based on polarisation microscopy called PolScope. Further, we investigate the mechanical behaviour by oocyte indentation. The oocyte is hold by a pipette and is then compressed at a force sensor. Combining the results from the different characterisation techniques, we can provide parameters sets for a mechanical model. A Finite element framework is used to study strains and stresses of the oocytes during cell compression.
The proposed mechanical model serves us as an additional tool to support in vitro fertilization research. After a gentle testing, it may be possible to get information about healthiness and stage of maturity, to raise the rate of successful fertilizations. So the results of mechanical tests could be used as an additional source of information to prevent an implantation of unsuitable oocytes in the female organism.
2019
A. E. Ehret, M. Böl
Recent topics in biomechanics and mechanobiology
GAMM Mitteilungen, 1, (2019) [Link]
J. Dittmann, S. Tesche, R. Krull, M. Böl
The influence of salt-enhanced cultivation on the micromechanical behaviour of filamentous pellets
Biochemical Engineering Journal, 148, 65-76, (2019) [Link]
A. Stracuzzi, J. Dittmann, M. Böl, A. E. Ehret
Visco- and poro-elastic contributions of the zona pellucida to the mechanical response of oocytes
submitted, (2019)
2018
J. Dittmann, A. Dietzel, M. Böl
Mechanical characterisation of oocytes - the influence of sample geometry on parameter identification
Journal of the Mechanical Behavior of Biomedical Materials, 77, 764-775, (2018) [Link]
2016
J. Dittmann, D. Töpfer, M. Böl
Experimental-based material parameter identification of oocytes
Proceedings in Applied Mathematics and Mechanics, 16, 79-80, (2016) [Link]
2015
M. Garcés-Schröder, M. Leester-Schädel, M. Schulz, M. Böl, A. Dietzel
Micro-Gripper: A new concept for a monolithic single-cell manipulation device
Sensors and Actuators A: Physical, 236, 130-139, (2015) [Link]
2018
M. Böl
Biomechanics - Multi-scale/field experimental approaches
University of Kassel, Kassel (Germany), April 2018
2017
M. Böl
Advanced experiments on biological systems at different length scales
ETH Zurich, Zurich (Zwitzerland), December 2017
M. Böl, J. Dittmann, D. Töpfer
On the mechanical characterisation of oocytes
Jahrestagung der Gesellschaft für Angewandte Mathematik und Mechanik (GAMM), Weimar (Germany), March 2017
J. Dittmann, D. Töpfer, M. Böl
On the compressibility of the porcine zona pellucida
EUROMECH Colloquium 585, Warberg (Germany), February 2017
2016
M. Böl, J. Dittmann, D. Töpfer
On the experimental measurement of oocyte mechanical properties
The 12th World Congress of Computational Mechanics (WCCM XII), Seoul (South Korea), July 2016
M. Böl, J. Dittmann, D. Töpfer
On the mechanical testing of oocytes
European Congress on Computational Methods in Applied Sciences and Engineering (ECCOMAS), Crete Island (Greece), June 2016
J. Dittmann, D. Töpfer, M. Böl
Experimental based material parameter identification of oocytes
Jahrestagung der Gesellschaft für Angewandte Mathematik und Mechanik (GAMM), Braunschweig (Germany), March 2016
Mechanical characterisation of the stomach
The stomach is a part of the digestive tract connecting the esophagus and the duodenum. It is a J-shaped hollow organ consisting of four parts: stomach dome, stomach entrance, stomach body and stomach outlet. In these parts the stomach wall varies in thickness, folds and behavior of the active contraction in order to satisfy different tasks of the stomach such as storing and breaking up the ingested aliment.
In the empty state, the stomach has many folds, whose quantity and magnitude depend on the region. These folds disappear in the filled state. Stomach muscles ensure the mixture and the reforwarding of the aliment as well they guarantee a basic tonus at a low filling level already. The stomach peristalsis and emptying is a rhythmic contraction of the muscles and dependent of different parameters like volume, osmotic pressure, acid concentration and chemical composition. The sphincter muscles are responsible for the segmentation of the aliment. Because of its complex microstructure and its macroscopic geometry, the stomach is mechanically flexible which keeps a low internal pressure at different volumes.
To characterise the non-linear, viscoelastic and strongly anisotropic stomach tissue, various tests are performed on stomach strips. Active experiments include tests of the elastic properties, the proliferation of the potential and the dependency of stress-length, stress-velocity and activation-length of the stomach. To measure the stress-length dependency, the stomach strips or single layers are tested in longitudinal and transversal orientation at the same time via a biaxial testing machine. To measure the dispersion of the potential and its velocity, tissue strips are used which are prepared with electrodes at the mucosal and serous side on the transversal and longitudinal muscles. The contraction is provoked via electrodes at one end of the strip or via stretch. Because of surface electrodes the proliferation velocity and the direction of the potential can be measured. In these tests the deformation is controlled by an optical measuring system.
On the whole stomach, contraction measurements are executed to describe the pressure progress and the three-dimensional deformation. Therefore, three kinds of tests are performed: First, isovolumetric measurements of the internal pressure in relation of the given liquid volumes, second, liquid volume release at isobaric contractions at different pressures and third, combined internal pressure, potential and deformation measurements. To determine the spatial distribution of the potential during the contraction, electromyography in form of surface electrodes is applied. To describe the complex architecture of the stomach wall, histological examinations are performed in a space-resolved way. Especially the orientation of collagen and muscles and their distribution in different layers in loaded and unloaded conditions are of interest for understanding the behavior of the stomach.
With these test results, a phenomenological electro-chemo-mechanical model can be developed which displays the whole contraction process beginning from the electric stimulus and ending at the mechanical contraction response. This model is validated continuously with the ongoing examination.
2020
M. Bauer, E. Morales-Orcajo, L. Klemm, R. Seydewitz, V. Fiebach, T. Siebert, M. Böl
Biomechanical and microstructural characterisation of the porcine stomach wall: Location- and layer-dependent investigations
Acta Biomaterialia, 102, 83-99, (2020) [Link]
L. Klemm, R. Seydewitz, M. Borsdorf, T. Siebert, M. Böl
On a coupled electro-chemomechanical model of gastric smooth muscle contraction
Acta Biomaterialia, 109, 163-181, (2020) [Link]
2019
A. E. Ehret, M. Böl
Recent topics in biomechanics and mechanobiology
GAMM Mitteilungen, 1, (2019) [Link]
2017
A. Tomalka, M. Borsdorf, M. Böl, T. Siebert
Porcine stomach smooth muscle force depends on history-effects
Frontiers in Physiology – Gastrointestinal Sciences, 8, 802, (2017) [Link]
2019
L. Klemm, M. Bauer, E. Morales-Orcajo, R. Seydewitz, M. Böl
On the electro-chemo-mechanical modelling of stomach smooth muscle contraction
Jahrestagung der Gesellschaft für Angewandte Mathematik und Mechanik (GAMM), Vienna (Austria), February 2019
2018
M. Böl
Biomechanics - Multi-scale/field experimental approaches
University of Kassel, Kassel (Germany), April 2018
2017
M. Böl
Advanced experiments on biological systems at different length scales
ETH Zurich, Zurich (Zwitzerland), December 2017
Numerical modelling of inhomogeneous volume growth
Growth in living bodies is understood as the increase of mass and volume during their development. Depending on the directions in which volume growth takes place, the growth process can be equal in every direction (isotropic growth) or can appear with preferred directions (anisotropic growth). When loads or constraints interact with the body during growth, some growing bodies change their growth behaviour. More specifically, these bodies grow in a direction inducing a lower energy state, as if their growth behaviour is maintained. With this statement, one can find isotropic growing bodies that grow anisotropically, or anisotropically growing bodies that change their direction and properties due to the existence of loads/constrains.
Using a phenomenological point of view, these growing bodies seem to change their growing direction when boundaries constrain them. Hereby, it can be considered that during growth the body behaves like a viscoelastic fluid while the mechanical behaviour is typical of solid materials. The changes on the growth directions are described as the flow of a viscoelastic material by means of a flow rule. Some restrictions have to be imposed in the flow rule due to the solid behaviour of those materials and the consideration of a positive growth (the addition of material is always greater or equal to zero). In a micro scale, the reorientation process can be seen as an adaptive process of cell division in the direction of the elastic deformations or stresses.
The use of numerical models for the simulation of growth phenomena has been increasing in popularity in the last two decades. The prediction of the shape in growing bodies considering morphogenesis and reorientation of volume growth is a challenging objective in biomechanics. Growth models implemented into nonlinear finite element approaches allows the use of complex geometries and boundary conditions in the simulations. Therefore, the development of finite element models for analysis of growth phenomena presents a flexible tool for studies involving adaptive, morphological changes and reorientation in volume growth processes.
2019
A. E. Ehret, M. Böl
Recent topics in biomechanics and mechanobiology
GAMM Mitteilungen, 1, (2019) [Link]
2018
N. Beißner, A. Bolea Albero, J. Füller, T. Kellner, L. Lauterboeck, J. Liang, M. Böl, B. Glasmacher, C. Müller-Goymann, S. Reichl
Improved in vitro models for preclinical drug and formulation screening focusing on 2D and 3D skin and cornea constructs
European Journal of Pharmaceutics and Biopharmaceutics, 126, 57-66, (2018) [Link]
2017
T. Siebert, A. Tomalka, N. Stutzig, K. Leichsenring, M. Böl
Changes in three-dimensional muscle structure of rabbit gastrocnemius, flexor digitorum longus, and tibialis anterior during growth
Journal of the Mechanical Behavior of Biomedical Materials, 74, 507-519, (2017) [Link]
M. Böl, K. Leichsenring, T. Siebert
Effects of growth on muscle, tendon and aponeurosis tissues in rabbit shank musculature
The Anatomical Record, 300, 1123-1136, (2017) [Link]
2016
A. Bolea Albero, M. Böl
On the modelling of finite growth considering the mechanics of cell division
Proceedings in Applied Mathematics and Mechanics, 16, 925-928, (2016) [Link]
J. Liang, A. Bolea Albero, J. Füller, C. Müller-Goymann, M. Böl
Computational and experimental analysis of wound healing processes
Proceedings in Applied Mathematics and Mechanics, 16, 95-96, (2016) [Link]
2015
A. Bolea Albero, M. Böl
Mechanics of cell-division: A new continuum model for growth inhomogeneities
Proceedings in Applied Mathematics and Mechanics, 15, 85-86, (2015) [Link
2014
M. Böl, A. Bolea Albero
On a new model for inhomogeneous volume growth of elastic bodies
Journal of the Mechanical Behavior of Biomedical Materials, 29, 582-593, (2014) [Link]
A. Bolea Albero, A. E. Ehret, M. Böl
A new approach to the simulation of microbial biofilms by a theory of fluid-like pressure-restricted finite growth
Computer Methods in Applied Mechanics and Engineering, 272, 271-289, (2014) [Link]
A. Bolea Albero, M. Böl
Growing between barriers
Proceedings in Applied Mathematics and Mechanics, 14, 99-100, (2014) [Link]
2019
M. Böl
Skeletal muscle mechanics – experiment and simulation
Paris-Saclay University Biomechanics Seminar Series, École Polytechnique, Palaiseau (France), October 2019
2018
M. Böl
Biomechanics - Multi-scale/field experimental approaches
University of Kassel, Kassel (Germany), April 2018
2017
M. Böl
Advanced experiments on biological systems at different length scales
ETH Zurich, Zurich (Zwitzerland), December 2017
2016
A. Bolea Albero, J. Liang, M. Böl
Computational modelling of wound healing: Cell division and migration
5. Kolloquium Neuartige Synthese- und Formulierungsverfahren für schwerlösliche Arzneistoffe und empfindliche Biopharmazeutika (SynFoBiA), Braunschweig (Germany), October 2016
Numerical simulation of contraction in muscle packages
Aim of this research is the development and validation of a three-dimensional finite element muscle model for dynamic muscle contraction considering changes on the geometry of the muscle. Skeletal muscles are tight packed inside the body (e.g. calf) and interact with the surrounded muscle tissue, thus a free deformation is impossible. The (intramuscular) transfer of forces in longitudinal and transversal direction influences the force development and deformation of single muscles. Maybe this fact complicates muscle coordination and a sophisticated force development. However, in daily routine muscles have to accomplish complex movements. In order to get a deeper understanding of muscle packages under the premise of force and velocity generation, analyses of muscle architecture, three-dimensional deformation and force development of muscle packages are necessary.
Several experiments are carried out aiming to the identification of the active/passive mechanical properties and structural parameters of each muscle of the muscle package. A step-by-step approach is used in order to compute the specific model parameters at each scale, where the information of the previous steps is used for the computation of more complex model parameters. In order to compare the experimental and modelling data, the geometry of the muscle is recorded during contraction. Therefore, the motion of three-dimensional muscle geometries of isolated muscles is captured with a camera system. The reconstruction of the muscle geometries is realised first by hand and finally optimised for an automatic reconstruction of the muscle geometries during the whole contraction.
Three successive steps are needed for characterising the muscle package. First, experiment and simulation at the soleus muscle is realised. The muscle architecture, fibre distribution, and the contraction behaviour of the gastrocnemius muscle are much more complex compared to the soleus muscle. Having this in mind, in the second step the modelling approach is validated on experimental data of the gastrocnemius muscle. Finally, the third step focuses on the development and validation of the first three-dimensional model muscle package (soleus, gastrocnemius, and plantaris muscle). To model the interaction between neighbouring muscles, experiments are realised to specify the force transfer. A basic requirement for the validation process is the combination between the determination of muscle force and three-dimensional muscle shape deformation (using optical measurement systems) for single muscles as well as for the whole muscle package.
2020
P. Schenk, S. Papenkort, M. Böl, T. Siebert, R. Grassme, C. Rode
A simple geometrical model accounting for 3D muscle architectural changes across muscle lengths
Journal of Biomechanics, 103, 109694, (2020) [Link]
R. Rockenfeller, M. Günther, N. Stutzig, D. Haeufle, T. Siebert, S. Schmitt, K. Leichsenring, M. Böl, T. Götz
Exhaustion of skeletal muscle fibers within seconds: incorporating phosphate kinetics into a Hill-type model
Frontiers in Physiology, Striated Muscle Physiology, in press, (2020) [Link]
2019
A. E. Ehret, M. Böl
Recent topics in biomechanics and mechanobiology
GAMM Mitteilungen, 1, (2019) [Link]
R. Seydewitz, T. Siebert, M. Böl
On a three-dimensional constitutive model for history effects in skeletal muscles
Biomechanics and Modeling in Mechanobiology, 18, 1665-1681, (2019) [Link]
2018
C. Wick, M. Böl, Florian Müller, R. Blickhan, T. Siebert
Packing of muscles in the rabbit shank influences three-dimensional architecture of M. soleus
Journal of the Mechanical Behavior of Biomedical Materials, 83, 20-27, (2018) [Link]
2017
T. Siebert, A. Tomalka, N. Stutzig, K. Leichsenring, M. Böl
Changes in three-dimensional muscle structure of rabbit gastrocnemius, flexor digitorum longus, and tibialis anterior during growth
Journal of the Mechanical Behavior of Biomedical Materials, 74, 507-519, (2017) [Link]
M. Böl, K. Leichsenring, T. Siebert
Effects of growth on muscle, tendon and aponeurosis tissues in rabbit shank musculature
The Anatomical Record, 300, 1123-1136, (2017) [Link]
2016
M. Böl, K. Leichsenring, M. Ernst, A. E. Ehret
Long-term mechanical behaviour of skeletal muscle tissue in semi-confined compression experiments
Journal of the Mechanical Behavior of Biomedical Materials, 63, 115-124, (2016) [Link]
C. Weichert, K. Leichsenring, M. Ernst, C. Wick, T. Siebert, R. Blickhan, M. Böl
Three-dimensional reconstruction of M. gastrocnemius contraction
Proceedings in Applied Mathematics and Mechanics, 16, 111-112, (2016) [Link]
L. Reinhardt, T. Siebert, K. Leichsenring, R. Blickhan, M. Böl
Intermuscular pressure between synergistic muscles correlates with muscle force
Journal of Experimental Biology, 219, 2311-2319, (2016) [Link]
2015
M. Böl, K. Leichsenring, M. Ernst, C. Wick, R. Blickhan, T. Siebert
Novel microstructural findings in m. plantaris and their impact during active and passive loading at macro level
Journal of the Mechanical Behavior of Biomedical Materials, 51, 25-39, (2015) [Link]
M. Böl, A. E. Ehret, K. Leichsenring, M. Ernst
Tissue-scale anisotropy and compressibility of tendon in semi-confined compression tests
Journal of Biomechanics, 48, 1092-1098, (2015) [Link]
T. Siebert, K. Leichsenring, C. Rode, C. Wick, N. Stutzig, H. Schubert, R. Blickhan, M. Böl
Three-Dimensional Muscle Architecture and Comprehensive Dynamic Properties of Rabbit Gastrocnemius, Plantaris and Soleus: Input for Simulation Studies
PloS ONE, 10, e0130985 (online), (2015) [Link]
2014
M. Böl, A. E. Ehret, K. Leichsenring, C. Weichert, R. Kruse
On the anisotropy of skeletal muscle tissue under compression
Acta Biomaterialia, 10, 3225-3234, (2014) [Link]
2019
M. Böl
Skeletal muscle mechanics – experiment and simulation
Paris-Saclay University Biomechanics Seminar Series, École Polytechnique, Palaiseau (France), October 2019
M. Böl
Aspects of active muscle modelling - multi-scale/field modelling in biomechanics
International Autumn School 2019, Berlin (Germany), October 2019
M. Böl
Three-dimensional multiscale/field modelling in muscle mechanics - Challenges in modelling and experiment
24th Sports Science University Day of the DVS, Berlin (Germany), September 2019
2018
M. Böl
Biomechanics - Multi-scale/field experimental approaches
University of Kassel, Kassel (Germany), April 2018
2017
M. Böl
Advanced experiments on biological systems at different length scales
ETH Zurich, Zurich (Zwitzerland), December 2017
2013
M. Sturmat, C. Weichert, K. Leichsenring, T. Siebert, R. Blickhan, M. Böl
Skeletal Muscle Contraction – Experiment and Theory
18th International Symposium on Computational Biomechanics, Ulm (Germany), May 2013
Polymers: From single chains to continuum bodies
Overwhelmingly many materials are comprised of polymers. In these a vast amount of single polymer chains are connected by various mechanisms to form a three-dimensional network. Such polymeric materials can be found in both industrial products and nature. Motivated by its abundant occurrence the development of a material model for polymeric networks has been the aim of many efforts. To derive such a model based on microscopic effects one needs first to examine the mechanical behaviour of a single polymer chain.
In the last decades techniques have been developed to conduct tensile tests on single polymeric molecules. The target of currently ongoing research at the institute of solid mechanics is the development of a mechanistic model able of reproducing the results of these experiments. Eventually the knowledge of the mechanical behaviour of a single polymer chain can be used to deduce a model of a polymeric network. Further, we also aim to the degradation and erosion in polymers. The modelling is motivated by a micromechanical description. Since degradation leads to the erosion of polymers, the modelling approach will consider the dependency of both effects. Here, degradation is modelled as a chemo mechanical problem. In order to simulate degradation and erosion in complex geometries, the modelling approach is embedded into a finite element framework.
2020
J. Liu, A. Mildner, J. Großeheilmann, M. Böl
Polymerized Ionic Liquids (PILs)-based hydrogels with extremely high mechanical strength
submitted, (2020)
2019
M. Schulz, J. Dittmann, M. Böl
Modeling the mechanical behavior of semi-flexible polymer chains using a surrogate model based on a finite-element approach to Brownian polymer dynamics
Journal of the Mechanics and Physics of Solids, 130, 101-117, (2019) [Link]
M. Schulz, M.Böl
A finite element formulation for a geometrically exact Kirchhoff-Love beam based on constrained translation
Computational Mechanics, 64, 1155-1175, (2019) [Link]
2017
M. Schulz, M. Böl
An objective and locking-free finite-element formulation for geometrically exact Kirchhoff rods
Proceedings in Applied Mathematics and Mechanics, 17, 347-348, (2017) [Link]
2016
M. Schulz, M. Böl
Modeling the quasi-static mechanical behavior of the extensible worm-like chain in the finite-element framework
Proceedings in Applied Mathematics and Mechanics, 16, 393-394, (2016) [Link]
2014
J. Hellriegel, S. Günther, I. Kampen, A. Bolea Albero, A. Kwade, M. Böl, R. Krull
A biomimetic gellan-based hydrogel as a physicochemical biofilm model
Journal of Biomaterials and Nanobiotechnology, 5, 83-97, (2014) [Link]
R. Seydewitz, A. Bolea Albero, M. Böl
A continuum mechanics approach for modelling arbitrary degradation and erosion processes in polymers
Proceedings in Applied Mathematics and Mechanics, 14, 395-396, (2014) [Link]
2018
M. Böl
Biomechanics - Multi-scale/field experimental approaches
University of Kassel, Kassel (Germany), April 2018
2017
M. Böl
Advanced experiments on biological systems at different length scales
ETH Zurich, Zurich (Zwitzerland), December 2017
M. Schulz, M. Böl
An objective and locking-free finite-element formulation for geometrically exact Kirchhoff rods
Jahrestagung der Gesellschaft für Angewandte Mathematik und Mechanik (GAMM), Weimar (Germany), March 2017
From the muscle fibre to muscle tissue – experiments, modelling and simulation at micro, meso, and macro scale
The present project deals with the analysis of complex deformation states of skeletal muscle tissue whose results will be integrated in a numerical multi-scale modelling approach. The essential aim is the determination of the mechanical behaviour of the extracellular matrix (ECM) and consequently the prediction of changes based on quantitative and/or structural changes of the ECM. This knowledge, however, is of high medical as well as socioeconomical importance but rooted on previous analytical methods it could not be obtained. In order to advance research on this field, the present project combines two areas of expertise: microsystems technology and solid mechanics. This unique combination presents a completely new and innovative approach and enables the development of new methods in order to analyse skeletal muscle tissue from the experimental as well as numerical point of view.
Therefore, this project provides extensive experimental analyses on different size scales. In a first step we apply at micro scale (10-90 μm) axial tension as well as axial/transversal compression experiments on single muscle fibres and muscle fibres segments, respectively. Further, at meso scale (0.1-1 mm), we focus on compression and shear-compression tests applied on cubes excised from muscle fascicles. Finally, cubes resected from muscle tissue will be tested at macro scale (1-24 mm).
In order to perform such experiments special setups at micro and meso scale need to be developed. Their main components will be micro technological parallel grippers. In doing so, single muscle fibres or cubes resected from fascicles by means of ultra-short-pulse laser can be loaded and forces, displacements and deformations can be measured. Experiments at macro scale will be accomplished using well-known experimental methods.
Based on these multi-scale experiments and by means of the inverse finite element method we establish a three-step identification process that allows to determinate the mechanical behaviour of ECM. Finally, rooted on these findings a multi-scale modelling approach and its validation is scheduled.
2020
R. Rockenfeller, M. Günther, N. Stutzig, D. Haeufle, T. Siebert, S. Schmitt, K. Leichsenring, M. Böl, T. Götz
Exhaustion of skeletal muscle fibers within seconds: incorporating phosphate kinetics into a Hill-type model
Frontiers in Physiology, Striated Muscle Physiology, in press, (2020) [Link]
M. Böl, R. Iyer, M. Garces-Schröder, S. Kohn, A. Dietzel
Mechano-geometrical skeletal muscle fibre characterisation under cyclic and relaxation loading
Journal of the Mechanical Behavior of Biomedical Materials, 101, 104001, (2020) [Link]
S. Kohn, K. Leichsenring, R. Kuravi, A. E. Ehret, M. Böl
Direct measurement of the direction-dependent mechanical behaviour of skeletal muscle extracellular
submitted, (2020)
R. Kuravi, K. Leichsenring, M. Böl, A. E. Ehret
3D finite element models from serial section histology of skeletal muscle tissue - The role of micro-architecture on mechanical behavior
submitted, (2020)
2019
A. E. Ehret, M. Böl
Recent topics in biomechanics and mechanobiology
GAMM Mitteilungen, 1, (2019) [Link]
M. Garcés-Schröder, T. Zimmermann, C. Siemers, M. Leester-Schädel, M. Böl, A. Dietzel
Shape memory alloy actuators for silicon microgrippers
Journal of Microelectromechanical Systems, 28, 869-881, (2019) [Link]
M. Böl, R. Iyer, J. Dittmann, M. Garcés-Schröder, A. Dietzel
Investigating the passive mechanical behaviour of skeletal muscle fibres: Micromechanical experiments and Bayesian hierarchical modelling
Acta Biomaterialia, 92, 277-289, (2019) [Link]
2018
C. Wick, M. Böl, Florian Müller, R. Blickhan, T. Siebert
Packing of muscles in the rabbit shank influences three-dimensional architecture of M. soleus
Journal of the Mechanical Behavior of Biomedical Materials, 83, 20-27, (2018) [Link]
M. Garcés-Schröder, D. Metz, L. Hecht, M. Leester-Schädel, R. Iyer, M. Böl, A. Dietzel
Characterization of skeletal muscle passive mechanical properties by novel micro-force sensor and tissue micro-dissection by femtosecond laser ablation
Microelectronic Engineering, 192, 70-76, (2018) [Link]
2019
M. Böl
Skeletal muscle mechanics – experiment and simulation
Paris-Saclay University Biomechanics Seminar Series, École Polytechnique, Palaiseau (France), October 2019
M. Böl
Aspects of active muscle modelling - multi-scale/field modelling in biomechanics
International Autumn School 2019, Berlin (Germany), October 2019
M. Böl
Three-dimensional multiscale/field modelling in muscle mechanics - Challenges in modelling and experiment
24th Sports Science University Day of the DVS, Berlin (Germany), September 2019
M. Böl
Multiscale/field modelling in muscle mechanics - experiment versus simulation
Technical University of Munich, Munich (Germany), February 2019
2018
M. Böl
Biomechanics - Multi-scale/field experimental approaches
University of Kassel, Kassel (Germany), April 2018
2017
M. Böl
Advanced experiments on biological systems at different length scales
ETH Zurich, Zurich (Zwitzerland), December 2017
The role of meso-scale structure on the mechanical response of soft musculoskeletal tissues
Muscle and tendon are particular bulky musculoskeletal tissues with a pronounced structural similarity: a hierarchical tubular architecture and a predominantly uniaxial alignment of elongated structures along a preferred direction. This distinguishes them in terms of structure from other tissues in the body. The response of both tissues to compressive loads has gained little attention compared to their tensile behaviour although both tissues are subjected to compressive states in-vivo. Recent work revealed a particular anisotropy in the compressive response of these tissues and suggests an important role of the hierarchical organisation of extracellular matrix structures in stabilising the fibre-like components. These observations help improving the understanding of load transfer in these tissues and have implications for modelling and simulation.
The main aim is a comprehensive understanding of the structural features and mechanisms that govern the peculiar response of musculoskeletal tissues to multiaxial loads. To this end, the role of meso-scale structures formed by the extracellular matrix for the mechanical behaviour of muscle and tendon tissues is investigated in detail, with a focus on several asymmetries between the responses to tensile and compressive loads. A comprehensive set of dedicated experiments on different length-scales is needed to determine non-linear tissue and meso-scale material properties under multiaxial loads. Histological tissue sections will be prepared under different loads and used to build computer models for multi-scale finite element simulations. In-situ experiments will be performed based on non-linear optical microscopy to connect the change of meso-structure with loads applied on tissue-scale. Finally, the experimental results together with the understanding gained from detailed finite element computations can be used to define a class of continuum constitutive models suitable to represent the non-linear anisotropic behaviour of musculoskeletal tissues.
2020
R. Rockenfeller, M. Günther, N. Stutzig, D. Haeufle, T. Siebert, S. Schmitt, K. Leichsenring, M. Böl, T. Götz
Exhaustion of skeletal muscle fibers within seconds: incorporating phosphate kinetics into a Hill-type model
Frontiers in Physiology, Striated Muscle Physiology, in press, (2020) [Link]
S. Kohn, K. Leichsenring, R. Kuravi, A. E. Ehret, M. Böl
Direct measurement of the direction-dependent mechanical behaviour of skeletal muscle extracellular
submitted, (2020)
S. Papenkort, M. Böl, T. Siebert
Three-dimensional muscle architecture of rabbit M. soleus during growth
submitted, (2020)
R. Kuravi, K. Leichsenring, M. Böl, A. E. Ehret
3D finite element models from serial section histology of skeletal muscle tissue - The role of micro-architecture on mechanical behavior
submitted, (2020)
2019
A. E. Ehret, M. Böl
Recent topics in biomechanics and mechanobiology
GAMM Mitteilungen, 1, (2019) [Link]
M. Böl, R. Iyer, J. Dittmann, M. Garcés-Schröder, A. Dietzel
Investigating the passive mechanical behaviour of skeletal muscle fibres: Micromechanical experiments and Bayesian hierarchical modelling
Acta Biomaterialia, 92, 277-289, (2019) [Link]
R. Seydewitz, T. Siebert, M. Böl
On a three-dimensional constitutive model for history effects in skeletal muscles
Biomechanics and Modeling in Mechanobiology, 18, 1665-1681, (2019) [Link]
2018
C. Wick, M. Böl, Florian Müller, R. Blickhan, T. Siebert
Packing of muscles in the rabbit shank influences three-dimensional architecture of M. soleus
Journal of the Mechanical Behavior of Biomedical Materials, 83, 20-27, (2018) [Link]
2017
M. Garcés-Schröder, L. Hecht, A. Vierheller, M. Leester-Schädel, M. Böl, A. Dietzel
Micro-grippers with femtosecond-laser machined in-plane agonist-antagonist SMA actuators integrated on wafer-level by galvanic riveting
Proceedings, 1 (4), 1-5, (2017) [Link]
2019
M. Böl
Skeletal muscle mechanics – experiment and simulation
Paris-Saclay University Biomechanics Seminar Series, École Polytechnique, Palaiseau (France), October 2019
M. Böl
Aspects of active muscle modelling - multi-scale/field modelling in biomechanics
International Autumn School 2019, Berlin (Germany), October 2019
M. Böl
Three-dimensional multiscale/field modelling in muscle mechanics - Challenges in modelling and experiment
24th Sports Science University Day of the DVS, Berlin (Germany), September 2019
R. Kuravi, K. Leichsenring, M. Böl, A. E. Ehret
Studying skeletal muscle tissue response with 3D virtual samples reconstructed from histology
25th Congress of the European Society of Biomechanics, Vienna (Austria), July 2019
M. Böl
Multiscale/field modelling in muscle mechanics - experiment versus simulation
Technical University of Munich, Munich (Germany), February 2019
R. Kuravi, A. Oswald, K. Leichsenring, M. Böl, A. E. Ehret
Histology-based semi-automated 3D reconstruction and simulation of skeletal muscle tissue
Jahrestagung der Gesellschaft für Angewandte Mathematik und Mechanik (GAMM), Vienna (Austria), February 2019
2018
K. Leichsenring, A. Ehret, M. Böl
Load transfer mechanisms in skeletal muscle – influence of connective tissue on the mechanical behaviour
8th World Congress of Biomechanics, Dublin (Ireland), July 2018
M. Böl
Biomechanics - Multi-scale/field experimental approaches
University of Kassel, Kassel (Germany), April 2018
2017
M. Böl
Advanced experiments on biological systems at different length scales
ETH Zurich, Zurich (Zwitzerland), December 2017
Cleaning mechanisms of immersed systems
In future the offer of fresh water in many regions on earth runs short. Various studies predict an increase of 55% in water consumption. The highest increases is expected in the manufacturing sector, the thermal electricity generation, and in private household. Especially in food industry and pharmaceutical industry as part of the processing industry the majority of water consumption is used for cleaning processes. Based on the combination of available optimisation potential of current cleaning processes, rooted on a better understanding of effect mechanisms and the huge increase of water consumption, there exists a substantial cost-saving options of the water resources.
However, cleaning processes of fouled organic material on heat transfer surfacesisa complex interaction of interfacial interactions, mass transfer, heat transfer, fluid forces and chemical reactions, whichis presently not fully understood. The main objective in this research project is to achieve a fundamental knowledge of the cause-effect relationships in the cleaning of immersed systems. Wheyprotein in the form of Whey Protein Isolate (WPI) gel are to be used as a representative model fouling system. The research project is divided into three parts. The first part comprises the investigation of all material properties which are relevant for a cleaning process, specifically strength, viscoelastic characteristics, diffusion coefficient, surface topography, adhesion and cohesion force. These material parameters will be used in the second part of the project. Here a fundamental database for a continuum-mechanical finite element model of the fouling layer will be set up. The fluid phase will be simulated with a fluid-structure interaction model (FSI) model, so that the cleaning process of WPI can be simulated. To validate and improve the FSI model, two established methods, namely fluid dynamic gauging (FDG) and local phosphorescence detection (LPD), are used in the third part. The FDG method enables to investigate the three stages of the cleaning process, swelling stage, uniform stage and decay stage. In combination with a particle size analyser the particle size of removed particles can be determined. With the LPD method, it is possible to locally determine the cleaning time in different flow geometries under laminar and turbulent flow conditions. By correlation of the specific light intensity and the fouling layer thickness a removal rate as well as a change of the layer thickness can be quantified. The modelling approach will be developed further and based on this FSI model a tailored and system-specific cleaning strategy can be designed and validated. Furthermore well-established methodological approaches will be available, which can then be applied to any fouling layer.
2020
J. Liu, H. Wiese, W. Augustin, S. Scholl, M. Böl
Mechanical comparison of milk and whey protein isolate fouling deposits using indentation testings
Food and Bioproducts Processing, 122, 145-158, (2020) [Link]
J. Liu, M. Helbig, J.-P. Majschak, M. Böl
Whey protein gel - experimental testing and modelling of wire cutting
submitted, (2020)
2019
J. Liu, H. Wiese, S. Scholl, W. Augustin, M. Böl
Comparison of the mechanical properties of milk fouling and whey protein fouling
Jahrestagung der Gesellschaft für Angewandte Mathematik und Mechanik (GAMM), Vienna (Austria), February 2019
2018
J. Liu, H. Wiese, J. Dittmann, W. Augustin, S. Scholl, M. Böl
A comparison of the mechanical constitutive behaviour of milk protein deposits and fouling deposits from raw milk
Fouling and Cleaning in Food Processing, Lund (Sweden), April
2018
M. Böl
Biomechanics - Multi-scale/field experimental approaches
University of Kassel, Kassel (Germany), April 2018
2017
M. Böl
Advanced experiments on biological systems at different length scales
ETH Zurich, Zurich (Zwitzerland), December 2017
The urinary bladder is a central organ of vertebrates. Due to the extreme deformations on the entire bladder tissue and especially the active smooth muscle tissue needs to meet special requirements. Although there are many dysfunctions connected to tissue changes, mechanical analyses are not so common. The development of an electro-chemo-mechanical numerical model based on experimental investigations can be used as a tool for a better understanding of the living system.
To this end, experiments on bladders of domestic pigs are conducted, as their structure and contraction behaviour are similar to those of humans. Using a three-dimensional optical measurement technique a procedure is established to measure the bladder geometry in physiological saline solution. Furthermore, the measurement of the propagation of the action potential during contraction at the bladder’s surface is performed with surface electrode. The different layers of the bladder exhibit different architectures as well as active and passive characteristics that need to be determined. The data is used for an interim validation and a parameter identification of the electro-chemo-mechanical model.
The model developed in the scope of this project can be considered as a compensational method for animal models and, based on the similarity between human and porcine bladders, for human studies. Thus, in future it can contribute to a reduction of animal experiments and can help to have a more comprehensive understanding of bladder functions. Prospective applications of the model could deal with the prediction of different functional impacts of tissue changes (i.e. cicatrisation of the muscle layer due to interstitial cystitis). Therefore, morphological and mechanical changes of the sickened tissue have to be determined experimentally and incorporated into the model.
2020
R. Trostorf, E. Morales-Orcajo, T. Siebert, M. Böl
Location- and layer-dependent biomechanical and microstructural characterisation of the porcine urinary bladder wall
submitted, (2020)
2019
A. E. Ehret, M. Böl
Recent topics in biomechanics and mechanobiology
GAMM Mitteilungen, 1, (2019) [Link]
M. Borsdorf, A. Tomalka, N. Stutzig, E. Morales Orcajo, M. Böl, T. Siebert
Locational and directional dependencies of smooth muscle properties in pig urinary bladder
Frontiers in Physiology, 10, 63, (2019) [Link]
2018
E. Morales Orcajo, T. Siebert, M. Böl
Location-dependent correlation between tissue structure and the mechanical behaviour of the urinary bladder
Acta Biomaterialia, 75, 263-278, (2018) [Link]
2017
R. Seydewitz, E. Morales-Orcajo, M. Böl
Modelling the contraction properties of smooth muscle cells in bladder tissue
Proceedings in Applied Mathematics and Mechanics, 17, 223-224, (2017) [Link]
R. Seydewitz, R. Menzel, T. Siebert, M. Böl
Three-dimensional mechano-electrochemical model for smooth muscle contraction of the urinary bladder
Journal of the Mechanical Behavior of Biomedical Materials, 75, 128-146, (2017) [Link]
R. Menzel, M. Böl, T. Siebert
Importance of contraction history on muscle force of porcine urinary bladder smooth muscle tissue
International Urology and Nephrology, 49, 205-214, (2017) [Link]
2016
R. Seydewitz, M. Böl
On an electrical-chemical-mechanical approach for modelling smooth muscle excitation in bladder tissue
Proceedings in Applied Mathematics and Mechanics, 16, 393-394, (2016) [Link]
2018
E. Morales Orcajo, R. Seydewitz, T. Siebert, M Böl
Three-Dimensional Model for Smooth Muscle Contraction: Urinary Bladder Wall Application
13th World Congress in Computational Mechanics, New York City (USA), July 2018
R. Seydewitz, E. Morales-Orcajo, T. Siebert, M Böl
On a three-dimensional mechano-electrochemical model for smooth muscle
8th World Congress of Biomechanics, Dublin (Ireland), July 2018
E. Morales Orcajo, R. Seydewitz, T. Siebert, M. Böl
On the three-dimensional modelling for urinary bladder smooth muscle contraction
6th European Conference on Computational Mechanics (ECCM 6), Glasgow (UK), June 2018
M. Böl
Biomechanics - Multi-scale/field experimental approaches
University of Kassel, Kassel (Germany), April 2018
2017
M. Böl
Advanced experiments on biological systems at different length scales
ETH Zurich, Zurich (Zwitzerland), December 2017
R. Seydewitz, E. Morales Orcajo, T. Siebert, M. Böl
Modelling the contraction properties of smooth muscle cells in bladder tissue
Jahrestagung der Gesellschaft für Angewandte Mathematik und Mechanik (GAMM), Weimar (Germany), March 2017
E. Morales Orcajo, R. Seydewitz, R. Menzel, T. Siebert, M. Böl
Urinary bladder – experimental characterisation and numerical modelling
EUROMECH Colloquium 585, Warberg (Germany), February 2017
The present research deals with the analysis of complex deformation states of skeletal muscle tissue whose results are being integrated in a numerical multi-scale modelling approach. The essential aim is the determination of the mechanical behaviour of the extracellular matrix (ECM) and consequently the prediction of changes based on quantitative and structural changes of the ECM. This knowledge, however, is of high medical as well as socioeconomical importance but rooted on previous analytical methods it could not be obtained. In order to advance research on this field, two areas of expertise are combined: microsystems technology and solid mechanics. This unique combination presents a completely new and innovative approach and enables the development of new methods in order to analyse skeletal muscle tissue from the experimental as well as numerical point of view.
Therefore, it is provided extensive experimental analyses on different size scales. In a first step we apply at micro scale axial tension as well as axial/transversal compression experiments on single muscle fibres and muscle fibres segments, respectively. Further, at meso scale, we focus on compression and shear-compression tests applied on cubes excised from muscle fascicles. Finally, cubes resected from muscle tissue are tested at macro scale.
In order to perform such experiments special setups at micro and meso scale are developed. Their main components are micro technological parallel grippers. In doing so, single muscle fibres or cubes resected from fascicles by means of ultra-short-pulse laser can be loaded and forces, displacements and deformations can be measured. Experiments at macro scale are accomplished using experimental methods. Based on these multi-scale experiments and by means of the inverse finite element method an identification process is establish in order to determinate the mechanical behaviour of ECM. Finally, rooted on these findings a multi-scale modelling approach is validated.
2020
P. Schenk, S. Papenkort, M. Böl, T. Siebert, R. Grassme, C. Rode
A simple geometrical model accounting for 3D muscle architectural changes across muscle lengths
Journal of Biomechanics, 103, 109694, (2020) [Link]
R. Rockenfeller, M. Günther, N. Stutzig, D. Haeufle, T. Siebert, S. Schmitt, K. Leichsenring, M. Böl, T. Götz
Exhaustion of skeletal muscle fibers within seconds: incorporating phosphate kinetics into a Hill-type model
Frontiers in Physiology, Striated Muscle Physiology, in press, (2020) [Link]
M. Böl, R. Iyer, M. Garces-Schröder, S. Kohn, A. Dietzel
Mechano-geometrical skeletal muscle fibre characterisation under cyclic and relaxation loading
Journal of the Mechanical Behavior of Biomedical Materials, in press, (2020) [Link]
S. Papenkort, M. Böl, T. Siebert
Three-dimensional muscle architecture of rabbit M. soleus during growth
submitted, (2020)
R. Kuravi, K. Leichsenring, M. Böl, A. E. Ehret
3D finite element models from serial section histology of skeletal muscle tissue - The role of micro-architecture on mechanical behavior
submitted, (2020)
2019
A. E. Ehret, M. Böl
Recent topics in biomechanics and mechanobiology
GAMM Mitteilungen, 1, (2019) [Link]
M. Böl, R. Iyer, J. Dittmann, M. Garcés-Schröder, A. Dietzel
Investigating the passive mechanical behaviour of skeletal muscle fibres: Micromechanical experiments and Bayesian hierarchical modelling
Acta Biomaterialia, 92, 277-289, (2019) [Link]
R. Seydewitz, T. Siebert, M. Böl
On a three-dimensional constitutive model for history effects in skeletal muscles
Biomechanics and Modeling in Mechanobiology, 18, 1665-1681, (2019) [Link]
2018
C. Wick, M. Böl, Florian Müller, R. Blickhan, T. Siebert
Packing of muscles in the rabbit shank influences three-dimensional architecture of M. soleus
Journal of the Mechanical Behavior of Biomedical Materials, 83, 20-27, (2018) [Link]
M. Garcés-Schröder, D. Metz, L. Hecht, M. Leester-Schädel, R. Iyer, M. Böl, A. Dietzel
Characterization of skeletal muscle passive mechanical properties by novel micro-force sensor and tissue micro-dissection by femtosecond laser ablation
Microelectronic Engineering, 192, 70-76, (2018) [Link]
2017
M. Garcés-Schröder, L. Hecht, A. Vierheller, M. Leester-Schädel, M. Böl, A. Dietzel
Micro-grippers with femtosecond-laser machined in-plane agonist-antagonist SMA actuators integrated on wafer-level by galvanic riveting
Proceedings, 1 (4), 1-5, (2017) [Link]
T. Siebert, A. Tomalka, N. Stutzig, K. Leichsenring, M. Böl
Changes in three-dimensional muscle structure of rabbit gastrocnemius, flexor digitorum longus, and tibialis anterior during growth
Journal of the Mechanical Behavior of Biomedical Materials, 74, 507-519, (2017) [Link]
M. Böl, K. Leichsenring, T. Siebert
Effects of growth on muscle, tendon and aponeurosis tissues in rabbit shank musculature
The Anatomical Record, 300, 1123-1136, (2017) [Link]
2016
M. Böl, K. Leichsenring, M. Ernst, A. E. Ehret
Long-term mechanical behaviour of skeletal muscle tissue in semi-confined compression experiments
Journal of the Mechanical Behavior of Biomedical Materials, 63, 115-124, (2016) [Link]
C. Weichert, K. Leichsenring, M. Ernst, C. Wick, T. Siebert, R. Blickhan, M. Böl
Three-dimensional reconstruction of M. gastrocnemius contraction
Proceedings in Applied Mathematics and Mechanics, 16, 111-112, (2016) [Link]
L. Reinhardt, T. Siebert, K. Leichsenring, R. Blickhan, M. Böl
Intermuscular pressure between synergistic muscles correlates with muscle force
Journal of Experimental Biology, 219, 2311-2319, (2016) [Link]
2015
M. Böl, K. Leichsenring, M. Ernst, C. Wick, R. Blickhan, T. Siebert
Novel microstructural findings in m. plantaris and their impact during active and passive loading at macro level
Journal of the Mechanical Behavior of Biomedical Materials, 51, 25-39, (2015) [Link]
M. Böl, A. E. Ehret, K. Leichsenring, M. Ernst
Tissue-scale anisotropy and compressibility of tendon in semi-confined compression tests
Journal of Biomechanics, 48, 1092-1098, (2015) [Link]
T. Siebert, K. Leichsenring, C. Rode, C. Wick, N. Stutzig, H. Schubert, R. Blickhan, M. Böl
Three-Dimensional Muscle Architecture and Comprehensive Dynamic Properties of Rabbit Gastrocnemius, Plantaris and Soleus: Input for Simulation Studies
PloS ONE, 10, e0130985 (online), (2015) [Link]
2014
M. Böl, A. E. Ehret, K. Leichsenring, C. Weichert, R. Kruse
On the anisotropy of skeletal muscle tissue under compression
Acta Biomaterialia, 10, 3225-3234, (2014) [Link]
2019
M. Böl
Skeletal muscle mechanics – experiment and simulation
Paris-Saclay University Biomechanics Seminar Series, École Polytechnique, Palaiseau (France), October 2019
M. Böl
Aspects of active muscle modelling - multi-scale/field modelling in biomechanics
International Autumn School 2019, Berlin (Germany), October 2019
M. Böl
Three-dimensional multiscale/field modelling in muscle mechanics - Challenges in modelling and experiment
24th Sports Science University Day of the DVS, Berlin (Germany), September 2019
M. Böl
Multiscale/field modelling in muscle mechanics - experiment versus simulation
Technical University of Munich, Munich (Germany), February 2019
2018
R. Seydewitz, T. Siebert, M.Böl
On the phenomenological modelling of history effects in skeletal muscles
13th World Congress in Computational Mechanics, New York City (USA), July 2018
M. Böl
Biomechanics - Multi-scale/field experimental approaches
University of Kassel, Kassel (Germany), April 2018
R. Seydewitz, T. Siebert, M. Böl
A phenomenological approach for modelling force enhancement and depression in skeletal muscle tissue
Jahrestagung der Gesellschaft für Angewandte Mathematik und Mechanik (GAMM), Munich (Germany), March 2018
R. Seydewitz, T. Siebert, M. Böl
Modelling force enhancement and force depression in skeletal muscle tissue
Norddeutsches Mechanik Kolloquium 2018, Braunschweig, January 2018
2017
M. Böl
Advanced experiments on biological systems at different length scales
ETH Zurich, Zurich (Zwitzerland), December 2017
T. Siebert, A. Tomalka, K. Leichsenring, M. Böl
Changes in three dimensional muscle structure during growth
EUROMECH Colloquium 585, Warberg (Germany), February 2017
K. Leichsenring, T. Siebert, M. Böl
Skeletal muscle growth in rabbit shank musculature - an experimental study
EUROMECH Colloquium 585, Warberg (Germany), February 2017
2016
A. Bolea Albero, M. Böl
Phenomenological modelling of force enhancement and force depression in skeletal muscle contraction
The 12th World Congress of Computational Mechanics (WCCM XII), Seoul (South Korea), July 2016
K. Leichsenring, T. Siebert, M. Böl
Experimental quantification of skeletal muscle growth in oryctolagus cuniculus
The 12th World Congress of Computational Mechanics (WCCM XII), Seoul (South Korea), July 2016
K. Leichsenring, T. Siebert, M. Böl
Muscle growth – an experimental study
European Congress on Computational Methods in Applied Sciences and Engineering (ECCOMAS), Crete Island (Greece), June 2016
2013
M. Sturmat, C. Weichert, K. Leichsenring, T. Siebert, R. Blickhan, M. Böl
Skeletal Muscle Contraction – Experiment and Theory
18th International Symposium on Computational Biomechanics, Ulm (Germany), May 2013
Technische Universität Braunschweig
Universitätsplatz 2
38106 Braunschweig
Postfach: 38092 Braunschweig
Telefon: +49 (0) 531 391-0